Polyamines are required for virulence in Salmonella enterica serovar Typhimurium.

PloS One
Lotte JelsbakJohn Elmerdahl Olsen

Abstract

Sensing and responding to environmental cues is a fundamental characteristic of bacterial physiology and virulence. Here we identify polyamines as novel environmental signals essential for virulence of Salmonella enterica serovar Typhimurium, a major intracellular pathogen and a model organism for studying typhoid fever. Central to its virulence are two major virulence loci Salmonella Pathogenicity Island 1 and 2 (SPI1 and SPI2). SPI1 promotes invasion of epithelial cells, whereas SPI2 enables S. Typhimurium to survive and proliferate within specialized compartments inside host cells. In this study, we show that an S. Typhimurium polyamine mutant is defective for invasion, intracellular survival, killing of the nematode Caenorhabditis elegans and systemic infection of the mouse model of typhoid fever. Virulence of the mutant could be restored by genetic complementation, and invasion and intracellular survival could, as well, be complemented by the addition of exogenous putrescine and spermidine to the bacterial cultures prior to infection. Interestingly, intracellular survival of the polyamine mutant was significantly enhanced above the wild type level by the addition of exogenous putrescine and spermidine to the bacterial cult...Continue Reading

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Citations

Jan 10, 2014·PloS One·Ana Herrero-FresnoRene S Hendriksen
May 3, 2014·BioMed Research International·Laurent DortetPatrice Nordmann
Dec 20, 2012·Journal of Biomedicine & Biotechnology·Judee Grace Nemeno-GuanzonJeong Ik Lee
Mar 10, 2016·BMC Microbiology·Emilie Søndberg, Lotte Jelsbak
Mar 2, 2016·Avian Pathology : Journal of the W.V.P.A·Jacqueline Boldrin de PaivaWanderley Dias da Silveira
Oct 21, 2015·Frontiers in Physiology·Tiffanie M NelsonCarl J Yeoman
Jul 23, 2013·International Journal of Medical Microbiology : IJMM·Maria Letizia Di MartinoGianni Prosseda
May 2, 2014·Veterinary Medicine International·Anu RahalAruna T Kumar
Nov 12, 2014·Infection and Immunity·Cecilia MartiniJean-Christophe Giard
May 20, 2015·BMC Microbiology·Mette Rørbæk GantzhornLine Elnif Thomsen
Feb 18, 2015·Frontiers in Cellular and Infection Microbiology·Thomas DandekarJanina Noster
Apr 18, 2013·PloS One·John Bradley GoforthEce Karatan
Aug 15, 2012·Proceedings of the National Academy of Sciences of the United States of America·Moria C Chambers, David S Schneider
Oct 29, 2017·World Journal of Microbiology & Biotechnology·Aslıhan Örs Gevrekci
May 2, 2018·Infection and Immunity·Graham G WillseyMatthew J Wargo
Jul 15, 2015·Applied and Environmental Microbiology·Panagiotis SapountzisMichael Poulsen
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Mar 7, 2020·Critical Reviews in Microbiology·Rajashri BanerjiSunil D Saroj
Apr 11, 2013·The Journal of Biological Chemistry·Shin KuriharaPhilip N Rather

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Methods Mentioned

BETA
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