Since their discovery by Antoni van Leeuwenhoek in 1678 until the recent development of transgenic mice expressing proteins altering polyamine levels in a tissue-specific manner, polyamines have been the object of intense research efforts which have shed light on several biological and pathological processes. From the discovery of a particular form of proteasome regulation of the catabolism of the key regulatory enzyme in their synthetic pathway, to the experimental cancer treatment or prevention with polyamine antagonists or inhibitors of the latter enzyme, a whole spectrum of interests can be revealed. Still, many aspects of their functions remain elusive and difficulties inherent in their analysis, which relies on sophisticated high-performance liquid chromatographic (HPLC) methods, and the lack of standardization; have hampered the transit from the research realm to the standard clinical laboratory domain. Their assay in biological fluids has been used for cancer diagnosis and for monitoring anticancer treatment. In this article, we attempt to provide an overview of polyamine structure, nutritional value, metabolism, and physiological roles. Next, we will summarize the main analytical methods on which we count, and finally ...Continue Reading
The effect of spermidine and spermine on proliferation in vitro of fibroblasts from normal and cystic fibrosis patients
Ornithine decarboxylase assay in human colorectal mucosa. Methodologic issues of importance to quality control
Rectal mucosal ornithine decarboxylase activity is not a useful marker of risk for colorectal neoplasia
Involvement of polyamines in pancreatic growth induced by dietary soyabean, lectin or trypsin inhibitors
Aminoguanidine blocks intestinal diamine oxidase (DAO) activity and enhances the intestinal adaptive response to resection in the rat
Molecular mechanics of the interactions of spermine with DNA: DNA bending as a result of ligand binding
Modulation of growth gene expression by selective alteration of polyamines in human colon carcinoma cells
Molecular dynamics of spermine-DNA interactions: sequence specificity and DNA bending for a simple ligand
Phase I trial of alpha-difluoromethyl ornithine (DFMO) and methylglyoxal bis (guanylhydrazone) (MGBG) in patients with advanced prostatic cancer
Acidic residues important for substrate binding and cofactor reactivity in eukaryotic ornithine decarboxylase identified by alanine scanning mutagenesis.
Deficiency of endogenous arginine synthesis provokes hypertension by exhausting substrate arginine for nitric oxide synthesis
Advanced glycation end products (AGEs) on the surface of diabetic erythrocytes bind to the vessel wall via a specific receptor inducing oxidant stress in the vasculature: a link between surface-associated AGEs and diabetic complications
Synthesis of a fluorescent derivatizing reagent, 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate, and its application for the analysis of hydrolysate amino acids via high-performance liquid chromatography
Intersubunit location of the active site of mammalian ornithine decarboxylase as determined by hybridization of site-directed mutants
In vivo, synergestic inhibition of MAT-LyLu rat prostatic adenocarcinoma growth by polyamine deprivation and low-dose cyclophosphamide
Pretherapeutic erythrocyte polyamine spermine levels discriminate high risk relapsing patients with M1 prostate carcinoma
Erythrocyte spermine levels: a prognostic parameter in childhood common acute lymphoblastic leukemia
Lysine-69 plays a key role in catalysis by ornithine decarboxylase through acceleration of the Schiff base formation, decarboxylation, and product release steps
Increased translation efficiency and antizyme-dependent stabilization of ornithine decarboxylase in amino acid-supplemented human colon adenocarcinoma cells, Caco-2
Overexpression of antizyme in the hearts of transgenic mice prevents the isoprenaline-induced increase in cardiac ornithine decarboxylase activity and polyamines, but does not prevent cardiac hypertrophy
Phase III trial of accelerated hyperfractionation with or without difluromethylornithine (DFMO) versus standard fractionated radiotherapy with or without DFMO for newly diagnosed patients with glioblastoma multiforme
Aminoguanidine ameliorates overexpression of prosclerotic growth factors and collagen deposition in experimental diabetic nephropathy
Antioxidant effect of polyamines on erythrocyte cell membrane lipoperoxidation after free-radical damage
Transgenic mouse models for studies of the role of polyamines in normal, hypertrophic and neoplastic growth
Determinants of proteasome recognition of ornithine decarboxylase, a ubiquitin-independent substrate
The polyamines spermine and spermidine protect proteins from structural and functional damage by AGE precursors: a new role for old molecules?
Glucose, glycation, and RAGE: implications for amplification of cellular dysfunction in diabetic nephropathy
Atmospheric pressure chemical ionization-mass spectrometry method to improve the determination of dansylated polyamines
p53-dependent NDRG1 expression induces inhibition of intestinal epithelial cell proliferation but not apoptosis after polyamine depletion
Combination effects of platinum drugs and N1, N11 diethylnorspermine on spermidine/spermine N1-acetyltransferase, polyamines and growth inhibition in A2780 human ovarian carcinoma cells and their oxaliplatin and cisplatin-resistant variants
The Physiologic Roles of the Subepithelial Platelet-derived Growth Factor Receptor α-positive Cells in the Colon (Am J Physiol Gastrointest Liver Physiol 2013;304:G823-G834)
Association of glycemic profiles with whole blood polyamine among middle-aged Japanese men: colorimetric assay using oat and barley seedling polyamine oxidase
Polyamines induce resistance to cationic peptide, aminoglycoside, and quinolone antibiotics in Pseudomonas aeruginosa PAO1
Improved electrotransformation and decreased antibiotic resistance of the cystic fibrosis pathogen Burkholderia cenocepacia strain J2315
The effect of difluoromethylornithine on decreasing prostate size and polyamines in men: results of a year-long phase IIb randomized placebo-controlled chemoprevention trial
Spermidine promotes human hair growth and is a novel modulator of human epithelial stem cell functions
Norspermidine and novel Pd(II) and Pt(II) polynuclear complexes of norspermidine as potential antineoplastic agents against breast cancer
Functional consequences of perturbing polyamine metabolism in the malaria parasite, Plasmodium falciparum
Coordination Reactions and Noncovalent Interactions of Polyamines with Nucleotides in Binary Systems and with Nucleotides and Copper(II) Ion in Ternary Systems
Stability and Solution Structure of Binary and Ternary Cu(II) Complexes with l-Glutamic Acid and Diamines as Well as Adducts in Metal-Free Systems in Aqueous Solution
Cytoplasmic expression of the JM403 antigen GlcA-GlcNH3+ on heparan sulfate glycosaminoglycan in mammary carcinomas--a novel proliferative biomarker for breast cancers with high malignancy
Alternative antiglycation mechanisms: are spermine and fructosamine-3-kinase part of a carbonyl damage control pathway?
Simultaneous determination of 10 kinds of biogenic amines in rat plasma using high-performance liquid chromatography coupled with fluorescence detection
Determination of Biogenic Amines in Seawater Using Capillary Electrophoresis with Capacitively Coupled Contactless Conductivity Detection
Performance of purified grape pomace as a fining agent to reduce the levels of some contaminants from wine
Homeostasis of glutathione is associated with polyamine-mediated β-lactam susceptibility in Acinetobacter baumannii ATCC 19606
Inhibition of polyamine formation antagonizes vascular smooth muscle cell proliferation and preserves the contractile phenotype
Determination of agmatine using isotope dilution UPLC-tandem mass spectrometry: application to the characterization of the arginine decarboxylase pathway in Pseudomonas aeruginosa
Serum metabolite profile associated with incident type 2 diabetes in Koreans: findings from the Korean Genome and Epidemiology Study
Evaluation of biogenic amines and nitrate in raw and pickled jurubeba (Solanum paniculatum L.) fruit
Does polyamine oxidase activity influence the oxidative metabolism of children who suffer of diabetes mellitus?
AMD1 is required for the maintenance of leukemic stem cells and promotes chronic myeloid leukemic growth.
Development of enzyme-linked immunosorbent assays for the determination of N1, N12-diacetylspermine in human urine
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