Polyamines in vaccinia virions and polypeptides released from viral cores by acid extraction.

Journal of Virology
W Lanzer, J A Holowczak

Abstract

Vaccinia virions propagated in the presence of [3H]ornithine were found to contain two labeled polyamines, spermine and spermidine. In complete virions the ratio of radioactively labeled spermine to spermidine was about 1:10, whereas in viral cores the ratio was 2:5. This suggests that some spermidine was preferentially lost during the conversion of virions to cores or that spermidine was present in the virions both inside and outside the core structure. Addition of [3H]ornithine to vaccinia virus-infected cells as late as 6 h postinfection demonstrated that, although the conversion of this precursor to polyamines was reduced by 50% or more as compared to mock-infected cells, complete inhibition of polyamine synthesis did not occur. Two percent or less of the total radioactivity associated with virions grown in the presence of [3H]ornithine was found to be acid soluble. Polyacrylamide gel electrophoretic analysis showed that all the structural polypeptides were labeled when virions were propagated in the presence of [3H]ornithine. When cores labeled with a mixture of 14C-labeled amino acids were extracted with 0.25 N H2SO4, 12 to 15% of the labeled core polypeptides were released and could be precipitated with acetone. About 40...Continue Reading

References

Mar 3, 1975·Biochemical and Biophysical Research Communications·J Hodgson, J D Williamson
Mar 1, 1974·The Journal of General Virology·U BachrachH Wiener
Sep 1, 1974·Journal of Virology·G Roening, J A Holowczak
Nov 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·W Gibson, B Roizman
Mar 1, 1966·Journal of Ultrastructure Research·K B Easterbrook
Aug 14, 1967·Journal of Molecular Biology·K I Oda, W K Joklik
Apr 11, 1958·Science·B N AMESS M ROSENTHAL
Apr 1, 1964·Proceedings of the National Academy of Sciences of the United States of America·Y BECKER, W K JOKLIK
Jun 1, 1962·Virology·M W JOHNSON, R MARKHAM
May 1, 1975·Journal of Virology·D M Pett, H S Ginsberg

❮ Previous
Next ❯

Citations

Sep 15, 2017·Microbiology and Molecular Biology Reviews : MMBR·Bryan C MounceJohn H Connor
Apr 25, 2020·Biomolecules·Mason R Firpo, Bryan C Mounce
Apr 21, 2018·International Journal of Molecular Sciences·Olga A SmirnovaAlexander V Ivanov
May 3, 2019·Journal of Virology·Vincent MastrodomenicoBryan C Mounce
Jul 21, 2020·ACS Infectious Diseases·Vincent MastrodomenicoBryan C Mounce
Apr 1, 1980·Journal of Virology·S L SheppardS M Boyle
Nov 13, 1985·Biochimica Et Biophysica Acta·N Moussatché
Aug 19, 2016·Journal of Virology·Bryan C MounceMarco Vignuzzi
Jan 1, 1977·Journal of Virology·D C Kelly, R M Elliott
Jan 30, 1979·Biochemical and Biophysical Research Communications·A S TymsJ D Williamson
Aug 2, 2021·Microbial Pathogenesis·S T R MoolamallaP K Vinod

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.