PMID: 9443076Jan 27, 1998Paper

Polyamines may regulate S-phase progression but not the dynamic changes of chromatin during the cell cycle

Journal of Cellular Biochemistry
J LaitinenE Hölttä

Abstract

Several studies suggest that polyamines may stabilize chromatin and play a role in its structural alterations. In line with this idea, we found here by chromatin precipitation and micrococcal nuclease (MNase) digestion analyses, that spermidine and spermine stabilize or condense the nucleosomal organization of chromatin in vitro. We then investigated the possible physiological role of polyamines in the nucleosomal organization of chromatin during the cell cycle in Chinese hamster ovary (CHO) cells deficient in ornithine decarboxylase (ODC) activity. An extended polyamine deprivation (for 4 days) was found to arrest 70% of the odc- cells in S phase. MNase digestion analyses revealed that these cells have a highly loosened and destabilized nucleosomal organization. However, no marked difference in the chromatin structure was detected between the control and polyamine-depleted cells following the synchronization of the cells at the S-phase. We also show in synchronized cells that polyamine deprivation retards the traverse of the cells through the S phase already in the first cell cycle. Depletion of polyamines had no significant effect on the nucleosomal organization of chromatin in G1-early S. The polyamine-deprived cells were al...Continue Reading

References

Apr 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·D H Russell, P J Stambrook
Jan 1, 1975·Somatic Cell Genetics·T EgeN R Ringertz
Jan 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·D Riley, H Weintraub
Jul 12, 1976·Biochemical and Biophysical Research Communications·O HebyC B Wilson
Jul 11, 1991·Nucleic Acids Research·L XiaoH R Matthews
Mar 1, 1990·Molecular and Cellular Biology·J L Feng, B Villeponteau
Dec 1, 1990·Experimental Cell Research·B KamińskaB Grzelakowska-Sztabert
Jan 1, 1988·Annual Review of Biochemistry·D S Gross, W T Garrard
Mar 1, 1986·The Biochemical Journal·A E Pegg
Aug 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·T A Chen, V G Allfrey
Jan 1, 1985·Annual Review of Genetics·J C EissenbergS C Elgin
Mar 1, 1985·Experimental Cell Research·D A PrenticeL R Gurley
Jan 1, 1981·Differentiation; Research in Biological Diversity·O Heby
Jan 1, 1984·Annual Review of Biochemistry·C W Tabor, H Tabor
Jan 1, 1982·Annual Review of Biochemistry·T Igo-KemenesH G Zachau
Jun 1, 1983·Cell Biology International Reports·P S SunkaraN J Prakash
Feb 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·J AllanH Gould
Aug 1, 1994·Cytometry·P PohjanpeltoS Knuutila
Aug 1, 1991·Trends in Cell Biology·A P Wolffe

❮ Previous
Next ❯

Citations

Feb 28, 2008·Histochemistry and Cell Biology·Masashi ShinKunio Fujiwara
Mar 19, 2013·The International Journal of Biochemistry & Cell Biology·Tomoko YamashitaKazuei Igarashi
Jun 1, 2005·Experimental Cell Research·Sima NasizadehLo Persson
Feb 5, 2003·The International Journal of Biochemistry & Cell Biology·Merja AuvinenErkki Hölttä
Nov 27, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Diana TetiHarold McNair
Oct 6, 2005·Journal of Cellular and Molecular Medicine·Nikolaus Seiler, Francis Raul
Mar 27, 2016·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Gustavo Petri GuerraCarlos Fernando Mello
Jan 26, 2010·Essays in Biochemistry·Kersti Alm, Stina Oredsson
Aug 18, 2000·Journal of Structural Biology·K Alm, S M Oredsson
Jun 19, 2012·Biochimica Et Biophysica Acta·Giuseppe IacominoLuciano D'Agostino
Aug 30, 2008·Biomacromolecules·C N N'soukpoé-KossiH A Tajmir-Riahi
Oct 1, 1998·Biochemical and Biophysical Research Communications·E GrassilliM A Desiderio
Oct 14, 2021·Langmuir : the ACS Journal of Surfaces and Colloids·Kiana S Sykes, Ryan J White

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Nature Cell Biology
Lionel Pintard, Matthias Peter
Journal of the American Society for Mass Spectrometry
Chagit Denekamp, Eisaam Egbaria
© 2021 Meta ULC. All rights reserved