PMID: 9428668Jan 15, 1998Paper

Polyamines regulate both transcription and translation of the gene encoding ornithine decarboxylase antizyme in mouse

European Journal of Biochemistry
J NilssonI Holm

Abstract

The degradation of ornithine decarboxylase (ODC) is mediated by antizyme, a protein regulated by the end-products of ODC activity, the polyamines. High levels of polyamines induce a +1 ribosomal frameshift in the translation of the rat antizyme message leading to the expression of a full-length protein. We have studied whether the regulation of antizyme expression occurs only at the level of translation or whether polyamine levels also affect the transcription of the antizyme gene. Thus, we have cloned and sequenced the mouse homologues of the rat ODC-antizyme gene and cDNA. Northern blot analysis shows that although high concentrations of polyamines do not affect the steady-state levels of antizyme message in L1210 leukemia cells, polyamine depletion using 2-(difluoromethyl)ornithine [Orn(F2Me)] leads to a marked decrease in mRNA levels. Results of transient transfections of luciferase-reporter-gene constructs driven by antizyme promoter fragments in untreated and Orn(F2Me)-treated Balb/C 3T3 cells indicate that the transcription of the antizyme gene is altered upon polyamine depletion. The amount of antizyme protein on Western blots was also altered by polyamine depletion and addition, and the polysomal distribution of antizy...Continue Reading

References

Jun 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·J S HellerE S Canellakis
Sep 1, 1990·Journal of Biochemistry·S MatsufujiS Hayashi
Apr 1, 1987·The Biochemical Journal·T Kameji, A E Pegg
Jan 1, 1981·Differentiation; Research in Biological Diversity·O Heby
Jun 15, 1982·The Biochemical Journal·K FujitaS Hayashi
May 17, 1995·Biochimica Et Biophysica Acta·T IchibaS Hayashi
Dec 14, 1994·Biochimica Et Biophysica Acta·D S TewariM Tewari
Feb 15, 1995·The Biochemical Journal·S Hayashi, Y Murakami
Sep 13, 1994·Proceedings of the National Academy of Sciences of the United States of America·T SuzukiK Igarashi
Apr 26, 1994·Proceedings of the National Academy of Sciences of the United States of America·E Rom, C Kahana
May 16, 1994·Biochemical and Biophysical Research Communications·T IchibaS Hayashi
Apr 1, 1993·European Journal of Biochemistry·Z Bercovich, C Kahana
Feb 16, 1996·The Journal of Biological Chemistry·Y MurakamiS Hayashi

❮ Previous
Next ❯

Citations

Feb 15, 2001·Journal of Cellular Physiology·E MatteucciM A Desiderio
Feb 26, 2013·Expert Reviews in Molecular Medicine·Shannon L NowotarskiRobert A Casero
Dec 14, 2011·The Journal of Biological Chemistry·Ramesh M RayLeonard R Johnson
Jan 30, 2004·Molecular and Cellular Biology·Jonas A NilssonJohn L Cleveland
Nov 7, 2009·PLoS Genetics·Keizo TokuhiroHiromitsu Tanaka
Apr 12, 2001·Plant Physiology·P BhatnagarS C Minocha
Feb 20, 2016·Scientific Reports·Ruanlin WangAihua Liang
Mar 10, 2001·Developmental Dynamics : an Official Publication of the American Association of Anatomists·A Gritli-LindeA Linde
May 4, 2007·American Journal of Physiology. Cell Physiology·Masato IsomeJoseph Satriano
Feb 7, 2006·The Journal of Biological Chemistry·Anthony E Pegg
Nov 12, 2020·Biochemistry·Dragana DespotovićDan S Tawfik
Oct 24, 2002·Biochimica Et Biophysica Acta·Tomasz HascilowiczYasuko Murakami

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.