Poly(beta-aminoester)s with pendant primary amines for efficient gene delivery

Bioconjugate Chemistry
Min LiuShi-Wen Huang

Abstract

Three hydrolytically degradable poly(beta-aminoester)s containing ester bonds in the main chain and primary amines in the side chain, synthesized by Michael polyaddition, were applied to deliver foreign DNA into cells in vitro. These linear polycations can condense DNA into small-sized particles with positive surface charge at high N/P ratios. Their high buffer capacity at pH 5-7 facilitated the escape of DNA from the endosome and resulted in efficient gene expression. Under the optimal conditions, poly(beta-aminoester)s with a pendant aminoethyl group showed higher transfection efficiencies than branched poly(ethylenimine) (PEI) 25KDa in 293T cells. The effect of side chain structure of the poly(beta-aminoester) on transfection efficiency has been investigated, which indicated that the poly(beta-aminoester) containing the pendant aminoethyl group was the most efficient carrier for both of 293T cells and COS-7 cells. The combination of hydrolytical degradation, high buffer capacity, relatively low cytotoxicity, and high transfection efficiency suggested that this kind of poly(beta-aminoester)s are novel promising nonviral gene carriers.

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Citations

Mar 28, 2013·ACS Applied Materials & Interfaces·Jun ChenMalcolm Mq Xing
Jul 28, 2010·Biotechnology Progress·Eun-Kyung LimSeungjoo Haam
Dec 27, 2011·Journal of Controlled Release : Official Journal of the Controlled Release Society·Wen-Tao HeRen-Xi Zhuo
Jan 26, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Kamal BauriPriyadarsi De
Dec 3, 2010·Nanoscale Research Letters·Min ZhangShi-Wen Huang
Feb 27, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Xiao-Ru WuXiao-Qi Yu
Oct 20, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·T J ThomasC K S Pillai

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