Polycarboxylated Dextran as a Multivalent Linker: Synthesis and Target Recognition of the Antibody-Nanoparticle Bioconjugates in PBS and Serum

Langmuir : the ACS Journal of Surfaces and Colloids
Filip KuncVladimir Gubala

Abstract

Nanoparticles (NPs) functionalized with antibodies on their surface are used in a wide range of research applications. However, the bioconjugation chemistry between the antibodies and the surface of nanoparticles can be very challenging, often accompanied by several undesired effects such as nanoparticle aggregation, antibody denaturation, or poor target recognition of the surface-bound antibodies. Here, we report on a synthesis of fluorescent silica nanoparticle-antibody (NP-Ab) conjugates, in which polycarboxylated dextran is used as the multivalent linker. First, we present a synthetic methodology to prepare polycarboxylated dextrans with molecular weights of 6, 40, and 70 kDa. Second, we used water-soluble, polycarboxylated dextrans as a multivalent spacers/linkers to immobilize antibodies onto fluorescent silica nanoparticles. The prepared NP-Ab conjugates were tested in a direct binding assay format in both phosphate-buffered saline buffer and whole serum to investigate the role of the spacer/linker in the capacity of the NP-Ab to specifically recognize their target in "clean" and also in complex media. We have compared the dextran conjugates with two standards: (a) NP-Ab with antibodies attached on the surface of nanopar...Continue Reading

References

Sep 8, 2004·Langmuir : the ACS Journal of Surfaces and Colloids·Rahul P BagweWeihong Tan
Apr 19, 2006·Langmuir : the ACS Journal of Surfaces and Colloids·Rahul P BagweWeihong Tan
Aug 10, 2007·Journal of Biomedical Materials Research. Part a·Yun-Xia SunRen-Xi Zhuo
Jun 28, 2008·Lab on a Chip·Christina JönssonJonas Melin
Sep 23, 2008·International Journal of Pharmaceutics·Jaleh VarshosazFarid Dorkoosh
Jun 9, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Nam Cao Hoai LeDavid E Williams
Aug 25, 2011·ACS Nano·Martin LundqvistKenneth Dawson
Nov 8, 2011·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Istvan Szelenyi
Jan 10, 2012·Analytical Chemistry·Vladimir GubalaDavid E Williams
Jul 7, 2012·Analytical Biochemistry·Andrew W DrakeScott L Klakamp
Jul 26, 2012·Biophysical Journal·Johannes HungerRalf P Richter
Oct 6, 2012·Advanced Drug Delivery Reviews·Vincent J Venditto, Francis C Szoka
Dec 6, 2012·Nature Nanotechnology·Marco P MonopoliKenneth A Dawson
Jan 23, 2013·The Analyst·Anke K TrillingHan Zuilhof
Mar 2, 2013·Nature Reviews. Drug Discovery·Rudy Juliano
May 29, 2015·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Giulia BattistelliMarco Montalti
Jun 10, 2015·Chemical Society Reviews·Thomas L MooreAlke Petri-Fink
Jun 13, 2015·Chemical Society Reviews·D DocterR H Stauber
Apr 19, 2017·Materials Science & Engineering. C, Materials for Biological Applications·Zhila ShaterabadiMeysam Soleymani
Jul 29, 2017·Chemical Reviews·Zdeněk FarkaPetr Skládal
Feb 27, 2018·Chemical Society Reviews·Christopher D SpicerMolly M Stevens
May 21, 2018·Colloids and Surfaces. B, Biointerfaces·Giorgia GiovaninniVladimir Gubala
Jun 10, 2018·Trends in Molecular Medicine·Gregory T TietjenJordan S Pober

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.