Polycation-induced benzoperylene probe excimer formation and the ratiometric detection of heparin and heparinase

Biosensors & Bioelectronics
Meiding YangCong Yu

Abstract

A benzoperylene probe excimer emission in an aqueous buffer solution is observed for the first time, and a novel ratiometric fluorescence method based on the probe excimer emission for the sensitive detection of heparin and heparinase is demonstrated. A negatively charged benzoperylene derivative, 6-(benzo[ghi]perylene-1,2-dicarboxylic imide-yl)hexanoic acid (BPDI), was employed. A polycation, poly(diallyldimethylammonium) chloride (poly-DDA), could induce aggregation of BPDI through noncovalent interactions. A decrease of BPDI monomer emission and a simultaneous increase of BPDI excimer emission were observed. Upon the addition of heparin, the strong binding between heparin and poly-DDA caused release of BPDI monomer molecules, and an excimer-monomer emission signal transition was detected. However, after the enzymatic hydrolysis of heparin by heparinase, heparin was hydrolyzed into small fragments, which weakened the competitive binding of heparin to poly-DDA. Poly-DDA induced aggregation of BPDI, and a monomer-excimer emission signal transition was detected. Our assay is simple, rapid, inexpensive, sensitive and selective, which could facilitate the heparin and heparinase related biochemical and biomedical research.

Citations

Apr 14, 2017·Chemical Communications : Chem Comm·Shiwu LiBen Zhong Tang
Feb 9, 2016·Chemical Communications : Chem Comm·Ching W Chan, David K Smith
Jul 12, 2018·Nanoscale·Salla VälimäkiMauri A Kostiainen
Oct 14, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Krishna Kalyani BeharaN D Pradeep Singh
Aug 28, 2017·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Meiding YangCong Yu
Oct 27, 2020·The Analyst·Liangfei FanYubin Ding
Oct 27, 2020·Analytical Methods : Advancing Methods and Applications·Weiqing LiCong Yu
Jul 28, 2021·Analytical Methods : Advancing Methods and Applications·Zhixiao HeHongyuan Yan
Oct 31, 2019·Langmuir : the ACS Journal of Surfaces and Colloids·Anup Kumar GhoshPrasanta Kumar Das
Nov 5, 2019·ACS Chemical Biology·Benjamin Ourri, Laurent Vial

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