PMID: 12787931Jun 6, 2003Paper

Polycation liposome-mediated gene transfer in vivo

Biochimica Et Biophysica Acta
Mitsuo MatsuuraNaoto Oku

Abstract

The polycation liposome (PCL), a recently developed gene transfer system, is simply prepared by a modification of liposomes with cetylated polyethylenimine (PEI), and shows remarkable transgene efficiency with low cytotoxicity. In the present study, we investigated the applicability of PCLs for in vivo gene transfer, since the PCL-mediated transgene efficiency was found to be maintained in the presence of serum. PCLs composed of dioleoylphosphatidylethanolamine (DOPE) with 5 mol% cetyl PEI (PEI average mr. wt. 1800), were superior for transfection to those of dipalmitoylphosphatidylcholine (DPPC) and cholesterol (2:1 as molar ratio) with 5 mol% cetyl PEI in vitro, although the latter PCLs were more efficient for gene transfer in vivo. PCL-DNA complexes were injected into mice via a tail or the portal vein, with the DNA being a plasmid encoding green fluorescent protein (GFP) or luciferase; and the expression was monitored qualitatively or quantitatively, respectively. Tail vein injection resulted in high expression of both GFP and luciferase genes in lung, and portal vein injection resulted in high expression of both genes in the liver. Concerning the gene delivery efficiency, the PCL was found to be superior to PEI or cetyl PE...Continue Reading

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Citations

Feb 3, 2005·Nature Reviews. Drug Discovery·Vladimir P Torchilin
Dec 5, 2012·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Hidenori AndoNaoto Oku
Feb 20, 2016·Journal of Controlled Release : Official Journal of the Controlled Release Society·Reatul KarimGeraldine Piel
Nov 15, 2012·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Koldo UrbiolaConchita Tros de Ilarduya
Oct 25, 2011·Journal of Controlled Release : Official Journal of the Controlled Release Society·Norihito YonenagaNaoto Oku
Apr 23, 2014·The Journal of Gene Medicine·Chia-Ling ChanCyrus R Safinya
Feb 1, 2007·Expert Opinion on Drug Discovery·Junichiro FutamiHidenori Yamada
Aug 31, 2006·Expert Opinion on Emerging Drugs·Matthieu D Lavigne, Dariusz C Górecki
Feb 6, 2004·Biochimica Et Biophysica Acta·Mayu SugiyamaNaoto Oku
Dec 25, 2012·International Journal of Pharmaceutics·Naozumi OhnishiHirofumi Takeuchi
Jun 24, 2008·Biochimica Et Biophysica Acta·Changfeng Chen, Carl P Tripp
Aug 25, 2016·Nanomedicine : Nanotechnology, Biology, and Medicine·Alexander EweAchim Aigner
Mar 19, 2014·The Journal of Pharmacy and Pharmacology·Joel G SouzaRenata F V Lopez
Jul 23, 2004·Expert Opinion on Biological Therapy·J Kent Leach, David J Mooney
Jun 16, 2004·Journal of Controlled Release : Official Journal of the Controlled Release Society·Yoshito TakeuchiNaoto Oku
Nov 5, 2008·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Kentaro HatanakaNaoto Oku
Jan 13, 2006·European Journal of Clinical Investigation·J P ChenC Lin
Oct 7, 2010·Journal of Microencapsulation·Conchita Tros de IlarduyaNejat Düzgünes
Oct 14, 2004·Nutrition Reviews·Javier CampiónJ Alfredo Martínez
May 11, 2004·Biological & Pharmaceutical Bulletin·Kosuke Shimizu, Naoto Oku
Oct 20, 2009·Bioconjugate Chemistry·Alexander PhilippArkadi Zintchenko
Oct 20, 2005·Journal of Bioscience and Bioengineering·Junichiro FutamiHidenori Yamada
Mar 3, 2011·Bioconjugate Chemistry·Tomohiro AsaiNaoto Oku
May 27, 2015·Chemical Reviews·Bhushan S PattniVladimir P Torchilin
Jul 21, 2006·The Journal of Physical Chemistry. B·Cuihong JiangCarl P Tripp

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