Polychromatic microarrays: simultaneous multicolor array hybridization of eight samples

Analytical Chemistry
Jason R E Shepard

Abstract

High-throughput microscale platforms have transformed modern analytical investigations. Traditional microarray analyses involve a comparative approach, with two samples, a known control and an unknown sample, hybridized side-by-side and then contrasted for genetic differences. The samples are labeled with separate dyes and hybridized together, providing a differential expression pattern based on the reporter intensities. In contrast, the fiber-optic microarray platform described herein is analyzed with a microscope, thereby enabling the use of virtually any reporter, including quantum dots. The instrumentation takes advantage of the narrow emission bands characteristic of quantum dots to perform multiplexed detection of Bacillus anthracis. Advancing beyond the standard red/green microarray experiment, a panel of eight reporters were linked to eight B. anthracis samples and simultaneously analyzed in a microarray format. The ability to employ an assortment of reporters, along with the capacity to simultaneously hybridize eight samples confers an unprecedented flexibility to array-based analyses, providing a 4-fold increase in throughput over standard two-color assays.

References

Sep 25, 1998·Science·M BruchezA P Alivisatos
Sep 15, 2000·Journal of Immunological Methods·N Baumgarth, M Roederer
May 3, 2002·Analytical Chemistry·Jason R EpsteinDavid R Walt
Jul 24, 2002·Nucleic Acids Research·Katarina LindroosAnn-Christine Syvänen
Feb 13, 2003·Nucleic Acids Research·M Juanita MartinezMargaret Werner-Washburne
Nov 6, 2003·Journal of the American Chemical Society·Jason R EpsteinDavid R Walt
Mar 30, 2004·Biopolymers·Gali SteinbergChanfeng Zhao
Aug 3, 2004·Nature Reviews. Immunology·Stephen P PerfettoMario Roederer
Sep 8, 2004·Trends in Cell Biology·Jyoti K Jaiswal, Sanford M Simon
Apr 20, 2005·Nature Genetics·Kevin L GundersonMark S Chee
May 28, 2005·Nature Genetics·Daniel Pinkel, Donna G Albertson
Jun 14, 2005·Nature Biotechnology·Yougen LiDan Luo
Aug 2, 2005·Nature Reviews. Cancer·Britta WeigeltLaura J van 't Veer
Sep 21, 2005·The Analyst·Peter D Wentzell, Tobias K Karakach

❮ Previous
Next ❯

Citations

Feb 22, 2008·Molecular Diagnosis & Therapy·Keith B HartmanMichael G Rosenblum
Feb 18, 2009·Analytical and Bioanalytical Chemistry·Wenwan Zhong
Aug 10, 2010·Analytical and Bioanalytical Chemistry·Maureen A WallingJason R E Shepard
Aug 9, 2008·International Journal of Nanomedicine·Igor L MedintzAaron R Clapp
Apr 1, 2009·International Journal of Molecular Sciences·Maureen A WallingJason R E Shepard
Mar 14, 2012·International Journal of Cancer. Journal International Du Cancer·Pericles PericleousEfstathios P Efstathopoulos
Jan 21, 2015·Colloids and Surfaces. B, Biointerfaces·K D NishaP Gunasekaran
Feb 7, 2014·Critical Reviews in Food Science and Nutrition·Avnesh Kumari, Sudesh Kumar Yadav
Apr 14, 2016·The Analyst·Qiang JuUlrich J Krull
Sep 30, 2010·Small·Caterina MinelliMolly M Stevens
Dec 22, 2007·Chemical Reviews·Audrey SassolasLoïc J Blum

❮ Previous
Next ❯

Related Concepts

Related Feeds

Anthrax Vaccines (ASM)

Three different types of anthrax vaccines are available; a live-attenuated, an alum-precipitated cell-free filtrate and a protein recombinant vaccine. The effectiveness between the three is uncertain, but the live-attenuated have shown to reduce the risk of anthrax with low adverse events. Here is the latest research on anthrax vaccines.

Anthrax

Anthrax toxin, comprising protective antigen, lethal factor, and oedema factor, is the major virulence factor of Bacillus anthracis, an agent that causes high mortality in humans and animals. Here is the latest research on Anthrax.

Anthrax Vaccines

Three different types of anthrax vaccines are available; a live-attenuated, an alum-precipitated cell-free filtrate and a protein recombinant vaccine. The effectiveness between the three is uncertain, but the live-attenuated have shown to reduce the risk of anthrax with low adverse events. Here is the latest research on anthrax vaccines.