Polyclonal B-cell activation by an arthritogenic Staphylococcus aureus strain: contribution of T-cells and monokines

Cellular Immunology
A Abdelnour, A Tarkowski

Abstract

We have recently described a murine model of Staphylococcus aureus-induced septic arthritis. One of the hallmarks of this disease is a striking hypergammaglobulinemia. In the present study we have used a sensitive ELISPOT technique to assess, at the single cell level, the B-cell differentiation properties of this arthritogenic, toxic shock syndrome toxin-1 (TSST-1)-producing staphylococcal strain. In vivo, inoculation of live S. aureus resulted in lymphoproliferation, early (within 3-4 days) peak of IgM-secreting cells and late (2 weeks after the injection) pronounced increase of IgG-secreting cells. We have documented that this late increase of IgG-secreting cells is a CD4+ T-cell-dependent phenomenon. Furthermore, we have showed that there is a relationship between the hypergammaglobulinemia and the appearance of arthritis, since a nonarthritogenic staphylococcal strain will not give rise to increased frequency of immunoglobulin-secreting cells. To elucidate mechanisms responsible for S. aureus-induced polyclonal B-cell activation, we have assessed in vitro effects of formalin-fixed arthritogenic S. aureus on the release of cytokines. Our results show that the S. aureus LS-1 strain induces in vitro preferentially IgM-secretin...Continue Reading

Citations

May 19, 2000·Infection and Immunity·M N RhemK R Wilhelmus
Jun 9, 2000·The Journal of Investigative Dermatology·L Mölne, A Tarkowski
Jan 22, 2008·FEMS Immunology and Medical Microbiology·Ann-Marie CalanderAndrej Tarkowski
Apr 2, 2003·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·M HolubO Beran
May 4, 2016·Proceedings of the National Academy of Sciences of the United States of America·Hwan Keun KimOlaf Schneewind
Mar 31, 2004·Microbes and Infection·Inger GjertssonAndrej Tarkowski
Oct 19, 2000·Arthritis and Rheumatism·M Verdrengh, A Tarkowski
Jul 5, 2002·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·R M Love, H F Jenkinson
Sep 1, 2012·Journal of Applied Physiology·Juliano M MartinsGlória E P Souza
Dec 11, 2002·Microbial Pathogenesis·Niklas PalmqvistElisabet Josefsson
Nov 1, 1996·Infection and Immunity·I M NilssonA Tarkowski

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