PMID: 12379356Oct 16, 2002

Polyclonal catalytic antibodies

Journal of Immunological Methods
Elizabeth L OstlerG Gallacher

Abstract

Polyclonal catalytic antibodies offer advantages in the evaluation of immunogens and in the ease of production of large quantities of antibodies. They comprise the entire immune response of an animal to an immunogen where monoclonals represent a subset. Polyclonal antibodies are consequently particularly suitable for evaluating catalytic antibody responses generated by different haptens or a group of structurally related haptens. The authors reported the first polyclonal catalytic antibodies in 1990. An unexpected finding is that polyclonal catalytic antibodies show single-site kinetic behaviour, i.e. whatever structural heterogeneity exists, the kinetic behaviour is homogeneous. Many groups worldwide have since published work in this area. Three groups are prominent. The authors' group, a group based in Austin, Texas, and led by Iverson, and a Shanghai group. The authors' group works with sheep antibodies and has published mechanistic studies and, more recently, specificity studies that revealed the catalysis of a beta-lactam. Most of this work over a 10-year period was performed by using a single bleed from a single sheep, which gives an indication of the ease of production and utility of such catalytic antibodies. Iverson's ...Continue Reading

References

Dec 19, 1986·Science·A TramontanoR A Lerner
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Jan 1, 1995·European Journal of Immunology·G Johnson, S W Moore
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May 29, 2000·Applied Biochemistry and Biotechnology·B AvalleD Thomas
Jun 22, 2000·Proceedings of the National Academy of Sciences of the United States of America·P VayronC Mioskowski
Nov 7, 2001·The Biochemical Journal·J WangM F Wilkinson

Citations

Sep 4, 2007·The Protein Journal·Xianghui MaJungang Fan
Oct 18, 2007·Chemistry Central Journal·Elizabeth L Ostler
Oct 4, 2005·Neurological Research·Matthew N Meriggioli
Sep 8, 2006·BJOG : an International Journal of Obstetrics and Gynaecology·A T N TitaS Dongmo

Related Concepts

Anti-Antibodies
Vaccination
Antibodies, Catalytic

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