Polycyclic Aromatic Hydrocarbons from Particulate Matter 2.5 (PM2.5) in Polluted Air Changes miRNA Profile Related to Cardiovascular Disease

Medical Science Monitor : International Medical Journal of Experimental and Clinical Research
Xiaonan HeShaoping Nie

Abstract

BACKGROUND Particulate matter 2.5 (PM2.5) in air pollution is regarded as a risk factor for cardiovascular disease (CVDs). Recently, it has become well accepted that polycyclic aromatic hydrocarbons (PAHs) in PM2.5 impacts human CVDs. However, few studies have shown miRNAs affected by PAHs play a critical role in transcriptional regulation related to cardiovascular development and disease. MATERIAL AND METHODS Human umbilical cord vein cells (HUVECs) incubated prior to treatment with PAHs at various concentrations (0, 100, 200, 300, 400, and 500 µg/ml) of PAHs particle solutions were added to the culture medium for 24 h. We performed isolation and sequencing of small RNAs and analysis of small RNA sequences and differential expression. The M3RNA database was used to predict miRNA-miRNA interactions. Tools from the DAVID database were used to perform the GO functional analysis of predicted miRNA target genes. A First-Strand cDNA Synthesis Kit was used to synthesis cDNA. RESULTS miRNA155 was revealed as a key regulator in PAHs treatment. The putative targets of upregulated miRNA in PAHs treatment indicated that the downregulated genes were enriched in biological pathways such as Wnt signaling and ErbB signaling, which are crucial...Continue Reading

Citations

Mar 19, 2019·Journal of Cellular Physiology·Zheng LiJianxiong Shen
Jul 10, 2019·International Journal of Environmental Research and Public Health·Indhumathi VeerappanRajaguru Palanisamy
Jul 30, 2020·International Journal of Environmental Health Research·B HermanovaR J Sram
Oct 23, 2019·Environmental Science and Pollution Research International·Zheng LiZhanyong Wu
Jun 3, 2021·International Journal of Environmental Research and Public Health·Po-Hsuan JengWei-Liang Chen
Jun 10, 2021·International Journal of Environmental Health Research·Zhixiang ZhouJingfeng Gao

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Methods Mentioned

BETA
RNA-seq

Software Mentioned

Microrna
Bowtie
Mireap
miRAlign
miRanda
predictive
DIANA
miRWalk
Mirtarget
DAVID

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