Polydatin mediates Parkin-dependent mitophagy and protects against mitochondria-dependent apoptosis in acute respiratory distress syndrome

Laboratory Investigation; a Journal of Technical Methods and Pathology
Tao LiYunfeng Li

Abstract

Mitophagy removes dysfunctional mitochondria and is known to play an important role in the pathogenesis of several diseases; however, the role of mitophagy in acute respiratory distress syndrome (ARDS) remains poorly understood. While we have previously demonstrated that polydatin (PD) improves lipopolysaccharide (LPS)-induced ARDS, the specific mechanism remains unclear. In present study, we aimed to determine whether PD activates Parkin-dependent mitophagy to protect against LPS-induced mitochondria-dependent apoptosis and lung injury. To establish the ARDS model, C57BL/6 mice were intratracheally injected with LPS (5 mg/kg) in vivo and Beas-2B cells were exposured to 0.5 mM LPS in vitro. Our results indicate that PD facilitates Parkin translocation to mitochondria and promotes mitophagy in ARDS-challenged mice and LPS-treated Beas-2B cells. However, PD-induced mitophagy was suppressed in Parkin-/- mice and Parkin siRNA transfected cells, indicating that PD activates Parkin-dependent mitophagy. Furthermore, the protective effects of PD against LPS-induced mitochondria-dependent apoptosis and lung injury were suppressed when Parkin was depleted both in vivo and in vitro. The inhibition of mitophagy with mitophagy inhibitor mit...Continue Reading

References

Oct 24, 2013·British Journal of Pharmacology·Rebecca E JimenezÅsa B Gustafsson
May 16, 2014·Respiratory Care·Tao LiZhongqing Chen
Aug 2, 2014·The Journal of Clinical Investigation·Kenji MizumuraAugustine M K Choi
Dec 3, 2014·Free Radical Biology & Medicine·Alan L ChangClaude A Piantadosi
Jun 23, 2015·The Journal of Surgical Research·Zhenhua ZengKe-seng Zhao
Apr 21, 2016·Free Radical Biology & Medicine·Baris Bingol, Morgan Sheng
Jul 13, 2016·American Journal of Physiology. Lung Cellular and Molecular Physiology·Saurabh AggarwalJianhua Zhang
Sep 7, 2016·Oncogene·J P BernardiniG Dewson
Sep 15, 2017·Cell Death Discovery·Xiaolan ZhuShuilong Leng
Jan 25, 2018·American Journal of Respiratory Cell and Molecular Biology·Takahiro HirumaYoshitsugu Yamada
Mar 24, 2018·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Chunxiao ZhangHongtao Yan

❮ Previous
Next ❯

Citations

Jul 21, 2020·Oxidative Medicine and Cellular Longevity·Xu LuoTao Li
Dec 29, 2020·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Lucas Amaral-MachadoEryvaldo S T Egito
Aug 15, 2021·Life Sciences·Archana SharmaMansoor Ali Syed
Dec 23, 2021·Phytotherapy Research : PTR·Penghui YeYaoguang Guo

❮ Previous
Next ❯

Methods Mentioned

BETA
transfection
X-ray

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Autophagy & Model Organisms

Autophagy is a cellular process that allows degradation by the lysosome of cytoplasmic components such as proteins or organelles. Here is the latest research on autophagy & model organisms