Polydimethylsiloxane materials with supraphysiological elasticity enable differentiation of myogenic cells

Journal of Biomedical Materials Research. Part a
Barbara Świerczek-LasekNatalia Krasteva

Abstract

Myogenic differentiation during muscle regeneration is guided by various physical and biochemical factors. Recently, substratum elasticity has gained attention as a physical signal that influences both cell differentiation and tissue regeneration. In this work, we investigated the influence of substratum elasticity on proliferation and differentiation of myogenic cells, mouse myoblasts of the C2C12 cell line and mouse primary myoblasts derived from satellite cells-muscle stem cells playing key role in muscle regeneration. Materials with different elastic moduli within the MPa scale based on polydimethylsiloxane (PDMS) were used as cell substratum and characterized for surface roughness, wettability, and micromechanical characteristics. We found that surface properties of PDMS substrates are alter nonlinearly with the increase of the material's elastic modulus. Using this system we provide an evidence that materials with elastic modulus higher than that of physiological skeletal muscle tissue do not perturb myogenic differentiation of both types of myoblasts; thus, can be used as biomaterials for muscle tissue engineering. PDMS materials with elasticity within the range of 2.5-4 MPa may transiently limit the proliferation of myo...Continue Reading

References

May 1, 1991·Journal of Biomechanical Engineering·C K Colton, E S Avgoustiniatos
Jul 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·H WeintraubA D Miller
Sep 19, 1997·Journal of Biomedical Materials Research·C R NunesL M Wolford
Oct 18, 2000·The Journal of Experimental Medicine·F LivakH T Petrie
Feb 1, 1961·The Journal of Biophysical and Biochemical Cytology·A MAURO
Oct 14, 2004·Journal of Biomedical Materials Research. Part a·George K ToworfePaul Ducheyne
Aug 23, 2006·Cell·Adam J EnglerDennis E Discher
Sep 2, 2006·Journal of Biomedical Materials Research. Part a·Bin LiJames H-C Wang
Jun 15, 2007·Tissue Engineering·Tanyarut BoontheekulDavid J Mooney
Aug 1, 2007·Journal of Materials Science. Materials in Medicine·Sandra TeixeiraFernando J Monteiro
Mar 5, 2009·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Kurt BoonenLiliane Schoofs
Oct 6, 2009·Journal of Biomechanics·Gwendolen C Reilly, Adam J Engler
Apr 15, 2010·Development·Tadanori Mammoto, Donald E Ingber
Oct 22, 2010·Pediatric Transplantation·Beniamino PalmieriLodi Daniele
Jun 2, 2011·Results and Problems in Cell Differentiation·Maria A CiemerychMarta Przewoźniak
Jul 5, 2011·Journal of Zhejiang University. Science. B·Razvan GhineaMaria del Mar Perez
Aug 25, 2012·The Journal of Contemporary Dental Practice·Eliseu Aldrighi MunchowCesar Henrique Zanchi
Nov 21, 2012·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Siddhi GuptaArvind Sinha
Apr 9, 2013·The FEBS Journal·Deborah Briggs, Jennifer E Morgan
Jun 8, 2013·Nature Communications·Anna UrciuoloPaolo Bonaldo
Jan 11, 2014·Biology of the Cell·Karolina ArchackaPaolo De Coppi
Jan 15, 2014·Biochimica Et Biophysica Acta·Francesca GattazzoPaolo Bonaldo
Oct 2, 2014·Advanced Drug Delivery Reviews·Christine A Cezar, David J Mooney
May 9, 2015·Critical Reviews in Eukaryotic Gene Expression·Anna BajekTomasz Drewa
May 29, 2015·Biotechnology, Biotechnological Equipment·Milena KeremidarskaNatalia Krasteva
Aug 4, 2015·Nature Biotechnology·Jérome ChalOlivier Pourquié
Nov 23, 2012·Science and Technology of Advanced Materials·Sara RomanazzoAkiyoshi Taniguchi
Jan 10, 2017·Journal of Tissue Engineering and Regenerative Medicine·Catherine G Y NganPeter F M Choong
Jun 3, 2017·Current Opinion in Biotechnology·Brian J Kwee, David J Mooney

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