PMID: 11904222Mar 21, 2002Paper

Polydispersity of Bacillus thuringiensis Cry1 toxins in solution and its effect on receptor binding kinetics

Biochimica Et Biophysica Acta
L MassonR Brousseau

Abstract

Dynamic light scattering and surface plasmon resonance techniques were used to investigate the influence of ionic strength, buffer composition and pH on the multimerization of trypsin-activated Cry1Ac and Cry1C toxins over time and the subsequent effects of the different multimers on receptor binding models. In carbonate buffer at pH 10.5, Cry1Ac and Cry1C assumed a monomeric state. After 24 h, a complete conversion of monomeric toxin to a dimeric or trimeric form was observed only for Cry1Ac under low ionic strength condition. Cry1C and Cry1Ac in high ionic strength buffer remained monomeric. Substitution of CAPS pH 11 for carbonate buffer suppressed this Cry1Ac oligomerization effect. Once Cry1Ac toxin was in an aggregated form, increases in ionic strength failed to revert the aggregated toxin back to a monomeric form. Monomeric Cry1Ac bound to a purified 115 kDa aminopeptidase N receptor from Manduca sexta in a 2:1 molar ratio thus confirming the existence of two binding sites on this receptor. Binding rates of dimeric or higher aggregated Cry1Ac toxin forms were different from those generated using the monomeric form and could not be fitted to existing binding models. In summary, our results confirm that the M. sexta 115 kD...Continue Reading

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Citations

Oct 19, 2011·The Journal of Biological Chemistry·Nicolas GroulxRikard Blunck
Jul 19, 2008·The Journal of Biological Chemistry·Manoj S Nair, Donald H Dean
Aug 21, 2014·Protein Science : a Publication of the Protein Society·Artem G EvdokimovSonya Franklin
Jan 21, 2004·Journal of Molecular Recognition : JMR·Rebecca L Rich, David G Myszka
Mar 18, 2004·Archives of Insect Biochemistry and Physiology·Edwin P AlcantaraMichael B Cohen
Nov 3, 2010·Environmental Science & Technology·Michael MadligerRené P Schwarzenbach
Apr 30, 2011·Environmental Science & Technology·Michael MadligerMichael Sander

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