Polydopamine-based molecularly imprinting polymers on magnetic nanoparticles for recognition and enrichment of ochratoxins prior to their determination by HPLC

Mikrochimica Acta
Meihua HuFangying Wu

Abstract

A polydopamine-based molecularly imprinted polymer was deposited on the surface of magnetite (ferroferric oxide) nanoparticles (Fe3O4@PDA MIPs) and is shown to be an efficient and fairly specific sorbent for the extraction of various ochratoxins. The MIPs were characterized by IR spectroscopy and transmission electron microscopy. The adsorption capacities, evaluated through the langmuir adsorption isotherm model, are 1.8, 0.23 and 0.17 mg·g-1 for ochratoxin A, ochratoxin B and ochratoxin C, respectively. Parameters such as the amount of magnetic MIPs, pH value, time for ultrasonication, elution solvent and volume were optimized. Following desorption from the MIP with acetonitrile, the ochratoxins were quantified by HPLC with fluorometric detection. Under optimal experimental conditions, the calibration plots are linear in the range of 0.01-1.0 ng·mL-1 of OTA, 0.02-2.0 ng·mL-1 of OTB, and 0.002-0.2 ng·mL-1 of OTC. The LODs are between 1.8 and 18 pg·mL-1, and the recoveries from spiked samples are 71.0% - 88.5%, with RSDs of 2.3-3.8% in case of rice and wine samples. The MIPs can be re-used for at least 7 times. Graphical abstract Schematic of the preparation of a magnetic molecularly imprinted polymer based on self-polymerizatio...Continue Reading

References

Apr 20, 2004·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Norbert M MaierWolfgang Lindner
May 16, 2014·Journal of Agricultural and Food Chemistry·Cristina GiovannoliClaudio Baggiani
Oct 11, 2016·Food Chemistry·Myra E Flores-Flores, Elena González-Peñas
May 24, 2017·Journal of Agricultural and Food Chemistry·Yaqiong ZhangHongtao Lei

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Citations

Feb 12, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Natalia CasadoIsabel Sierra
Feb 7, 2021·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Alireza KhataeeMir Reza Majidi

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