Polyelectrolyte multilayer films allow seeded human progenitor-derived endothelial cells to remain functional under shear stress in vitro

Acta Biomaterialia
N B ThébaudLaurence Bordenave

Abstract

There is considerable interest in making multilayer films for various applications, among which are cell contacting biomaterials, allowing new opportunities to prepare functionalized biomaterials. In this study we have explored the capability of poly(sodium-4-styrene sulfonate)/poly(allylamine hydrochloride) polyelectrolyte multilayer films (PMFs) as functional coatings for human progenitor-derived endothelial cells (PDECs), since the latter are a potential source of endothelial-type cells to be used in bioartificial vascular substitutes. We performed investigations with PDECs derived from peripheral blood and characterized as endothelial cells. After forming a confluent monolayer on PMFs they were exposed to laminar pulsatile physiological shear stress. We investigated whether PDECs were able to withstand shear stress and to respond at the mRNA (microarray analysis) and protein levels (thrombomodulin and tissue factor functional activity), in comparison with collagen I and fibrin glue used as controls. After shear stress the PDECs remained spread on the substrates, with a resulting increase in the number of expressed genes. Considering the functional significance of our findings for the regulation of coagulation and fibrinolyt...Continue Reading

Citations

Jan 8, 2011·Tissue Engineering. Part B, Reviews·Christopher J DetzelPadmavathy Rajagopalan
Feb 7, 2012·Biomaterials·Jeffrey T Krawiec, David A Vorp
Sep 24, 2013·Biomaterials·Murielle RémyLaurence Bordenave
Feb 13, 2019·Journal of Materials Science. Materials in Medicine·Olivia KérourédanRaphaël Devillard
Mar 15, 2020·Journal of Biomedical Materials Research. Part a·Caroline RoyerMarie-Christine Durrieu
May 9, 2015·The International Journal of Artificial Organs·Noélie B ThébaudLaurence Bordenave
Feb 27, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Michael J LandryChristopher J Barrett

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