Poly(ethylene glycol)--lipid conjugates regulate the calcium-induced fusion of liposomes composed of phosphatidylethanolamine and phosphatidylserine

Biochemistry
J W HollandT D Madden

Abstract

The effect of poly(ethylene glycol)--lipid (PEG--lipid) conjugates on liposomal fusion was investigated. Incorporation of PEG--lipids into large unilamellar vesicles (LUVs) composed of equimolar phosphatidylethanolamine (PE) and phosphatidylserine (PS) inhibited calcium-induced fusion. The degree of inhibition increased with increasing molar ratio of the PEG conjugate and with increasing size of the PEG moiety. Inhibition appeared to result from the steric barrier on the surface of the liposomes which opposed apposition of bilayers and interbilayer contact. In the presence of a large excess of neutral acceptor liposomes, however, fusogenic activity was restored. The rate of fusion under these conditions depended on the initial molar ratio of the PEG conjugate in the PE:PS vesicles and the length and degree of saturation of the acyl chains which composed the lipid anchor. These results are consistent with spontaneous transfer of the PEG--lipid from PE:PS LUVs to the neutral lipid sink reducing the steric barrier and allowing fusion of the PE:PS LUVs. The primary determinant of the rate of fusion was the rate of transfer of the PEG--lipid, indicating that liposomal fusion could be programmed by incorporation of appropriate PEG--l...Continue Reading

Citations

Jun 7, 2007·Pharmaceutical Research·Birgit RombergGert Storm
May 1, 2012·Pharmaceutical Research·Juliane NguyenFrancis Szoka
Apr 21, 1999·Biochimica Et Biophysica Acta·P PiresM C Pedroso de Lima
Nov 18, 2000·Progress in Lipid Research·D C DrummondJ Leroux
Apr 20, 2001·Advanced Drug Delivery Reviews·I M Hafez, P R Cullis
Dec 18, 2001·Advanced Drug Delivery Reviews·P Meers
Jun 6, 2000·Advanced Drug Delivery Reviews·P R Cullis, A Chonn
Jun 6, 2000·Advanced Drug Delivery Reviews·M C Woodle
Jun 6, 2000·Advanced Drug Delivery Reviews·M B BallyD L Reimer
Jun 6, 2000·Advanced Drug Delivery Reviews·O V GerasimovD H Thompson
Jun 6, 2000·Advanced Drug Delivery Reviews·E MastrobattistaG Storm
Jul 2, 2003·Biophysical Chemistry·Nill BergstrandKatarina Edwards
Apr 10, 2013·Molecular Therapy : the Journal of the American Society of Gene Therapy·Yu SakuraiHideyoshi Harashima
Dec 5, 2013·Molecular Therapy. Nucleic Acids·Ravi L RungtaBrian A Macvicar
Jul 10, 2012·Chemical Communications : Chem Comm·Adam J PlauntBradley D Smith
Nov 10, 2006·Critical Reviews in Food Science and Nutrition·Yan Xianghua, Xu Zirong
Mar 15, 2012·The AAPS Journal·Rupa R Sawant, Vladimir P Torchilin
Jan 1, 2009·Critical Reviews in Therapeutic Drug Carrier Systems·Anu PuriRobert Blumenthal
Jun 7, 2011·Annual Review of Biomedical Engineering·Leaf Huang, Yang Liu
Feb 17, 1998·Journal of Microencapsulation·M R Mozafari, V Hasirci
Oct 24, 2009·Journal of Liposome Research·Carla GasbarriAntonella Fontana
May 20, 1999·Molecular Membrane Biology·N MaurerP R Cullis
Dec 1, 2001·Expert Opinion on Biological Therapy·N MaurerP R Cullis
Jan 19, 2012·Expert Opinion on Drug Delivery·Marian E GindyJames J Cunningham
Feb 28, 2008·Drug Development and Industrial Pharmacy·Huan XuYi Lu
Nov 17, 2011·Journal of Pharmaceutical Sciences·Ayman El-SayedHideyoshi Harashima
Jan 5, 2016·Trends in Biotechnology·Andrew B HillCharles H Jones
Mar 1, 2011·Journal of Controlled Release : Official Journal of the Controlled Release Society·Khalid OumzilPhilippe Barthélémy

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