Polyethylene glycol and octa-arginine dual-functionalized nanographene oxide: an optimization for efficient nucleic acid delivery

Biomaterials Science
Rana ImaniShahab Faghihi

Abstract

The successful application of nucleic acid-based therapy for the treatment of various cancers is largely dependent on a safe and efficient delivery system. A dual-functionalized graphene oxide (GO)-based nanocarrier with the conjugation of aminated-polyethylene glycol (PEG-diamine) and octa-arginine (R8) for the intracellular delivery of nucleic acids is proposed. The functionalized sites are covalently co-conjugated and the PEG : R8 molar ratio is optimized at 10 : 1 to achieve a hydrocolloidally stable size of 252 ± 2.0 nm with an effective charge of +40.97 ± 1.05 and an amine-rich content of 10.87 ± 0.4 μmol g-1. The uptake of the nanocarrier in breast cancer cell lines, MCF-7 and MDA-MB 231, is investigated. The siRNA and pDNA condensation ability in the presence and absence of enzymes and the endosomal buffering capacity, as well as the intracellular localization of the gene/nanocarrier complex are also evaluated. Furthermore, the delivery of functional genes associated with the nanocarrier is assessed using c-Myc protein knockdown and EGFP gene expression. The effective uptake of the nanocarrier by the cells shows superior cytocompatibility, and protects the siRNA and pDNA against enzyme degradation while inhibiting their...Continue Reading

References

Dec 22, 1998·Molecular and Cellular Biology·C V Dang
Oct 6, 2000·Endocrine-related Cancer·D J Liao, R B Dickson
Oct 3, 2002·Nature Reviews. Cancer·Stella PelengarisGerard Evan
Nov 29, 2002·Gene Therapy·T Niidome, L Huang
Jan 18, 2003·Journal of Pharmaceutical Sciences·Christopher M Wiethoff, C Russell Middaugh
Mar 12, 2003·Advanced Drug Delivery Reviews·Hidenori OtsukaKazunori Kataoka
Jun 9, 2005·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·U LungwitzA Göpferich
Jul 25, 2006·Current Opinion in Pharmacology·Maarja Mäe, Ulo Langel
Jun 26, 2007·Journal of Controlled Release : Official Journal of the Controlled Release Society·Dana J GaryYou-Yeon Won
Jul 30, 2008·Journal of the American Chemical Society·Zhuang LiuHongjie Dai
Oct 8, 2009·Journal of Biomedical Nanotechnology·Li LiuRalph P Mason
Jun 15, 2010·The AAPS Journal·Jie WangJessie L-S Au
Nov 17, 2010·Journal of Controlled Release : Official Journal of the Controlled Release Society·Amir K VarkouhiHidde J Haisma
Sep 3, 2011·Breast Cancer Research : BCR·Deborah L Holliday, Valerie Speirs
Feb 9, 2012·Chemical Society Reviews·Rubul MoutVincent M Rotello
May 25, 2013·Advanced Healthcare Materials·Congyu WuJingyan Zhang
May 28, 2013·Acta Pharmacologica Sinica·Li-sha MaXia Li
Jul 11, 2013·Proceedings of the National Academy of Sciences of the United States of America·Yinfeng LiHuajian Gao
Jul 1, 2014·Artificial Cells, Nanomedicine, and Biotechnology·Elham AbbasiMorteza Milani
Dec 3, 2014·Acta Biomaterialia·Anh H NguyenTodd C McDevitt
Aug 21, 2016·Colloids and Surfaces. B, Biointerfaces·Rana ImaniShahab Faghihi
Oct 14, 2013·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Fang-Fang ChengJun-Jie Zhu

❮ Previous
Next ❯

Citations

Feb 6, 2019·Biomaterials Science·André F GirãoPaula A A P Marques
Nov 11, 2019·Angewandte Chemie·Shi GuoCécilia Ménard-Moyon
Feb 8, 2020·Chemistry : a European Journal·Isabella A VacchiCécilia Ménard-Moyon
Jul 16, 2020·International Journal of Molecular Sciences·Daniela CaccamoMaria Teresa Sciortino
Apr 8, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Ziyao KangKeliang Liu
Apr 5, 2019·Journal of Nanobiotechnology·Magdalena Bamburowicz-KlimkowskaIreneusz P Grudzinski
Jan 5, 2021·Applied Microbiology and Biotechnology·Kai MaTian-Yun Wang
Dec 19, 2020·Biomedicines·Saffiya HabibMoganavelli Singh
Aug 14, 2020·Molecular Pharmaceutics·Ester J KwonSangeeta N Bhatia
May 9, 2021·International Journal of Pharmaceutics·JinLing ZhengHongFei Chen
Jul 17, 2021·Journal of Nanobiotechnology·Guangman CuiGuangyu Yao

❮ Previous
Next ❯

Methods Mentioned

BETA
transfection
Assay
electrophoresis
fluorescence microscopy
AFM
confocal microscopy
gene knockdown
fluorescence

Software Mentioned

LSM
SPSS

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Cell Migration in Cancer and Metastasis

Migration of cancer cells into surrounding tissue and the vasculature is an initial step in tumor metastasis. Discover the latest research on cell migration in cancer and metastasis here.