Polyethylene glycol diacrylate-based supermacroporous monolithic cryogel as high-performance liquid chromatography stationary phase for protein and polymeric nanoparticle separation

Journal of Chromatography. a
Zhiyong ChenYuanzong Li

Abstract

A supermacroporous monolithic cryogel was directly prepared by in situ cryo-copolymerization in a stainless steel cartridge (70mmx5.0mm I.D.) using methacrylic acid (MAA) as functional monomer and polyethylene glycol diacrylate (PEGDA) as crosslinker. The highly crosslinked (90%, molar ratio) poly(MAA-PEGDA) cryogel had more uniform supermacropores with a mean diameter of 25microm compared to the poly(acrylamide)-based cryogels. The viability of poly(MAA-PEGDA) cryogel as a medium was demonstrated for separations of lysozyme from chicken egg white (CEW) and water-soluble poly(N-isopropylacrylamide-co-3-(dimethylamino) propyl methacrylamide) (NIPAM-DMAPMA) nanoparticles from its crude reaction solution. The dynamic binding capacities of lysozyme and the polymeric nanoparticles were 4.51x10(-3)micromol/ml and 33.4microg/ml, respectively. The lysozyme recovered from the above separations had a purity of more than 85%, and retained 90% of its enzymatic activity.

References

Nov 29, 2002·Journal of Chromatography. a·Pär ArvidssonBo Mattiasson
Jun 12, 2003·Journal of Virological Methods·Karmen BranovicRenata Mazuran
May 12, 2004·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·M Yakup AricaGülay Bayramoğlu
Sep 10, 2004·Journal of Separation Science·Fatima M PlievaBo Mattiasson
Jun 8, 2006·Chemical Communications : Chem Comm·Alan TomineyArno Kraft
Sep 15, 2006·Journal of Separation Science·Rangan Mallik, David S Hage
Nov 23, 2006·Journal of Chromatography. a·I V KalashnikovaT B Tennikova
Feb 20, 2007·Journal of Chromatography. a·Thomas H MoureyStephen T Balke

❮ Previous
Next ❯

Citations

Mar 11, 2010·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Tomoko MoriKen Hosoya
Jun 22, 2011·Biotechnology Progress·Noor Shad BibiMarcelo Fernández-Lahore
Jul 28, 2011·Journal of Separation Science·Jens Sproß, Andrea Sinz
Jun 8, 2011·Journal of Separation Science·Fatima M PlievaBo Mattiasson

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Cell Wall Structure

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Bacterial Cell Wall Structure (ASM)

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.