PMID: 107389Jan 5, 1979

Polyethylene-glycol induced fusion of bacterial protoplasts: direct selection of recombinants

Molecular & General Genetics : MGG
K Fodor, L Alföldi

Abstract

Direct selection for recombinants by supplemented minimal media from polyethylene-glycol (PEG)-induced fusion of protoplasts of polyauxotrophic strains of B. megaterium revealed striking physiological influences on the yield of recombinants. Cytoplasmic state of the protoplasts to be fused, rather than genetic events, determined the number of colonies obtained on the selection media. It is suggested that the physiological effects primarily influenced the ability of the fused protoplasts to revert to bacillary form.

References

Jan 1, 1975·Journal of Bacteriology·K FodorL Alföldi
Jun 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·K Fodor, L Alföldi
Jun 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·P SchaefferR D Hotchkiss

Citations

Jan 1, 1982·Molecular & General Genetics : MGG·C Sanchez-RivasP Schaeffer
Jan 1, 1980·Molecular & General Genetics : MGG·K RoståsL Alföldi
Mar 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·M Gabor, R D Hotchkiss
Jan 1, 1996·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·L Alföldi

Related Concepts

Bacillus megaterium
Bacteriological Techniques
Vigilon
Protoplasts
Recombination, Genetic

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