Polyethylene glycol inhibits intestinal neoplasia and induces epithelial apoptosis in Apc(min) mice

Cancer Letters
Hemant K RoyRamesh K Wali

Abstract

Efficacy of a safe and clinically utilized polyethylene glycol formulation (PEG-3350) to suppress intestinal tumors was investigated in the Apc(min) mouse-model of experimental carcinogenesis. Furthermore, based on our previous finding on the induction of apoptosis in HT-29 cells by PEG, we evaluated its ability to stimulate epithelial cell apoptosis in both Apc(min) mouse as well as AOM-treated rat as a potential molecular mechanism of chemoprevention. Twenty-two Apc(min) mice were randomized equally to PEG or vehicle (control) supplementation. Tumors were scored and uninvolved intestinal mucosal apoptosis was assayed using a modified terminal deoxynucleotidyl transferase-mediated nick end-labeling (TUNEL) assay and by immunohistochemical detection of cleaved caspase-3. Supplementation of Apc(min) mice with 10% PEG 3350 (in drinking water) resulted in a 48% (P<0.05) reduction in intestinal tumor burden and induced 2-3 fold increase in mucosal apoptosis. Dietary supplementation of polyethylene glycol (5%) also stimulated colonic mucosal apoptosis 4-5 fold in AOM-treated rats, the regimen that we previously reported to reduce tumor burden by 76% (P<0.05). In summary, we demonstrate, for the first time, that PEG does protect agai...Continue Reading

References

Apr 3, 2001·International Journal of Cancer. Journal International Du Cancer·G ParnaudL Gamet-Payrastre
Apr 17, 2001·International Journal of Cancer. Journal International Du Cancer·H K RoyA Ratashak
Mar 19, 2002·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Allen M Gown, Mark C Willingham
Sep 7, 2002·International Journal of Cancer. Journal International Du Cancer·Barry Iacopetta

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Citations

Oct 25, 2006·International Journal of Colorectal Disease·Sebastián VidelaJuan R Malagelada
May 18, 2006·Molecular Carcinogenesis·Hagit F YerushalmiEugene W Gerner
May 24, 2006·Scandinavian Journal of Gastroenterology·Sylviane TachéDenis E Corpet
Jul 23, 2013·American Journal of Physiology. Gastrointestinal and Liver Physiology·Mariëtte Y M van der WulpHenkjan J Verkade
Apr 29, 2015·International Journal of Cancer. Journal International Du Cancer·Angelo Pietro FemiaGiovanna Caderni
Oct 27, 2016·Cancer Prevention Research·Luai Al Rabadi, Raymond Bergan

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