PMID: 8605088Mar 1, 1996Paper

Polyethylene glycol modification of a galactosylated streptavidin clearing agent: effects on immunogenicity and clearance of a biotinylated anti-tumour antibody

British Journal of Cancer
D MarshallR H Begent

Abstract

Effective radioimmunotherapy is limited by slow antibody clearance from the circulation, which results in low tumour to blood ratios and restricts the dose of radiolabelled anti-tumour antibody that can be safely administrated. Avidin and streptavidin clearing agents have been shown to effectively complex and clear radioactive biotinylated antibodies from the circulation, but their immunogenicity may limit their repeated use. We have investigated whether polyethylene glycol (PEG) modification can reduce the immunogenicity of our galactosylated streptavidin (gal-streptavidin) clearing agent without altering its effectiveness as a clearing agent. The immune response evoked in mice after intraperitoneal infection of 30 micrograms of gal-streptavidin was decreased after PEG modification, as shown by lower antibody titres and a reduction in the number of mice that elicited an anti-gal-streptavidin response. The effect of PEG-modified gal-streptavidin on the blood clearance and tumour localisation of a 125I-labelled biotinylated anti-CEA was investigated in the LS174T human colon carcinoma xenograft in nude mice. Although PEG modified gal-streptavidin bound the [125I]biotinylated antibody in vivo, effective clearance from the circula...Continue Reading

Citations

Jun 16, 2010·Angiogenesis·Nirupama DeshpandeJürgen K Willmann
Jan 13, 2001·Cancer Biotherapy & Radiopharmaceuticals·S F Rosebrough, D F Hartley
Jan 1, 2008·Biomarker Insights·Hao HongWeibo Cai
Sep 16, 2014·Imaging in Medicine·Reshu Saini, Kenneth Hoyt
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Jun 1, 2007·Nanoscale Research Letters·Weibo CaiXiaoyuan Chen
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Jul 24, 2012·Radiology·Moritz F Kircher, Jürgen K Willmann
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Dec 6, 2018·ACS Nano·Weston J SmithDmitri Simberg
Sep 11, 2021·International Journal of Biological Macromolecules·Ernesto Lopes Pinheiro-JuniorEliane Candiani Arantes

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