PMID: 11933218Apr 5, 2002Paper

Polyethyleneimine-based immunopolyplex for targeted gene transfer in human lymphoma cell lines

The Journal of Gene Medicine
Vicent M GuillemSalvador F Aliño

Abstract

Specific and efficient delivery of genes into targeted cells is a priority objective in non-viral gene therapy. Polyethyleneimine-based polyplexes have been reported to be good non-viral transfection reagents. However, polyplex-mediated DNA delivery occurs through a non-specific mechanism. This article reports the construction of an immunopolyplex, a targeted non-viral vector based on a polyplex backbone, and its application in gene transfer over human lymphoma cell lines. Targeting elements (biotin-labeled antibodies), which should recognize a specific element of the target cell membrane and promote nucleic acid entry into the cell, were attached to the polyplex backbone through a bridge protein (streptavidin). Immunopolyplex transfection activity was studied in several hematological cell lines [Jurkat (CD3+/CD19-), Granta 519 (CD3-/ CD19+), and J.RT3-T3.5 (CD3-/CD19-)] using the EGFP gene as a reporter gene and anti-CD3 and anti-CD19 antibodies as targeting elements. Transfection activity was evaluated via green fluorescence per cell and the percentage of positive cells determined by flow cytometry. A significant selectivity of gene delivery was observed, since the anti-CD3 immunopolyplex worked only in Jurkat cells while the...Continue Reading

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Citations

Sep 11, 2002·Journal of Controlled Release : Official Journal of the Controlled Release Society·Vicent M GuillemSalvador F Aliño
Oct 17, 2007·Human Gene Therapy·Maki ShiotaRenu Wadhwa
Aug 29, 2003·Expert Opinion on Biological Therapy·Michael A BarryM Brandon Parrott
Feb 3, 2009·Journal of Immunological Methods·Stefanos TheoharisAndrew J T George
Jun 29, 2007·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Wei-Hong WenAn-Gang Yang
Feb 1, 2005·Journal of Controlled Release : Official Journal of the Controlled Release Society·Claudia StrehblowJean Bernhard Petri
Jun 16, 2004·Journal of Controlled Release : Official Journal of the Controlled Release Society·Shih-Jiuan ChiuRobert J Lee
Nov 18, 2005·Trends in Molecular Medicine·Hans-Peter Vornlocher
May 13, 2015·Molecular Therapy. Nucleic Acids·Breanne LandryHasan Uludag
Mar 2, 2018·Genes·Luis SendraSalvador F Aliño
Mar 9, 2010·Bioconjugate Chemistry·Shubiao ZhangBing Wang

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