Polyethyleneimine-polyethylene glycol copolymer targeted by anti-HER2 nanobody for specific delivery of transcriptionally targeted tBid containing construct

Artificial Cells, Nanomedicine, and Biotechnology
Batoul Saqafi, Fatemeh Rahbarizadeh

Abstract

The present research seeks to investigate the process of mixing targeted gene delivery and transcriptional targeting. We have conjugated Polyethylenimine polymers (PEI) and molecules of poly (ethylene glycol). The next step was covalent attachment of anti-HER2 variables domains of camelid heavy chains antibodies (VHHs) or nanobodies (Nbs) to the distal terminals of NHS-PEG3500 in PEI-PEG nanoparticles. The whole procedure yielded PEI-PEG-Nb immunoconjugates. Having determined the properties of polyplexes, steps were taken to investigate the most efficient ratio of PEI polymers to pDNA molecules (N/P) so that the greatest rate of transfection may be obtained. This immune targeted nano biopolymer could condense the gene constructs that coded a transcriptionally targeted truncated -Bid (tBid) killer gene which was controlled by the breast cancer-specific MUC1 promoter. The favourable physicochemical properties matching both the size and zeta potential were observed in engineered polyplexes. Elevated transfection efficiency in HER2 positive cell lines using Nb-modified polyplexes were shown by the results of flow cytometry as compared against non-modified particles. 1.6 and 4.8 fold higher transfection efficiencies were observed in...Continue Reading

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Citations

Aug 15, 2020·Frontiers in Oncology·Emily Y Yang, Khalid Shah
Jul 1, 2020·Frontiers in Bioengineering and Biotechnology·Zhenzhen ZhangXiang Zhou
Aug 23, 2020·International Journal of Molecular Sciences·Alberto JuanCarlos Alonso-Moreno
Aug 29, 2020·Pharmaceutics·Alberto JuanCarlos Alonso-Moreno
Jul 14, 2019·Seminars in Cancer Biology·Balney RajithaGanji Purnachandra Nagaraju
Jan 21, 2021·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Manman LiuYangzhong Liu
Jun 5, 2021·International Journal of Biological Macromolecules·Divya SharmaBuddhadev Layek
Jul 21, 2021·Bioconjugate Chemistry·Hichem MoulahoumSuna Timur
Aug 10, 2021·Recent Patents on Anti-cancer Drug Discovery·Sepideh KhaleghiShahryar K Nikkhoi

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