Polyethylenimine-mediated gene delivery into human bone marrow mesenchymal stem cells from patients.

Journal of Cellular and Molecular Medicine
Weiwei WangN Ma

Abstract

Transplantation of mesenchymal stem cells (MSCs) derived from adult bone marrow has been proposed as a potential therapeutic approach for post-infarction left ventricular (LV) dysfunction. However, age-related functional decline of stem cells has restricted their clinical benefits after transplantation into the infarcted myocardium. The limitations imposed on patient cells could be addressed by genetic modification of stem cells. This study was designed to improve our understanding of genetic modification of human bone marrow derived mesenchymal stem cells (hMSCs) by polyethylenimine (PEI, branched with Mw 25 kD), one of non-viral vectors that show promise in stem cell genetic modification, in the context of cardiac regeneration for patients. We optimized the PEI-mediated reporter gene transfection into hMSCs, evaluated whether transfection efficiency is associated with gender or age of the cell donors, analysed the influence of cell cycle on transfection and investigated the transfer of therapeutic vascular endothelial growth factor gene (VEGF). hMSCs were isolated from patients with cardiovascular disease aged from 41 to 85 years. Optimization of gene delivery to hMSCs was carried out based on the particle size of the PEI/DNA...Continue Reading

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Citations

Nov 15, 2011·Nanomedicine·Evgenya DelyaginaGustav Steinhoff
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Methods Mentioned

BETA
genetic modification
transfection
electrophoresis
fluorescence-activated cell sorter
fluorescence microscopy
FACS
ELISA
protein assay
flow cytometry
transfections

Software Mentioned

SPSS
FlowJo
Cell Cycle
BD

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