Polyfluorophore excimers and exciplexes as FRET donors in DNA.

Bioconjugate Chemistry
Yin Nah Teo, Eric T Kool

Abstract

We describe studies aimed at testing whether oligomeric exciplex and excimer fluorophores conjugated to DNA have the potential to act as donors for energy transfer by the Forster mechanism. Oligodeoxyfluorosides (ODFs) are composed of stacked, electronically interacting fluorophores replacing the bases on a DNA scaffold. The monomer chromophores in the twenty tetramer-length ODFs studied here include pyrene (Y), benzopyrene (B), perylene (E), dimethylaminostilbene (D), and a nonfluorescent spacer (S); these are conjugated in varied combinations at the 3' end of a 14mer DNA probe sequence. In the absence of an acceptor chromophore, many of the ODF-DNAs show broad, unstructured long-wavelength emission peaks characteristic of excimer and exciplex excited states, similar to what has been observed for unconjugated ODFs. Although such delocalized excited states have been widely studied, we know of no prior report of their use in FRET. We tested the ability of the twenty ODFs to donate energy to Cy5 and TAMRA dyes conjugated to a complementary strand of DNA, with these acceptors oriented either at the near or far end of the ODF-conjugated probes. Results showed that a number of the ODF fluorophores exhibited relatively efficient ener...Continue Reading

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Citations

Mar 20, 2012·Chemical Reviews·Yin Nah Teo, Eric T Kool
Mar 9, 2010·Chemical Reviews·Renatus W SinkeldamYitzhak Tor
May 8, 2010·Chemical Communications : Chem Comm·Nan DaiEric T Kool
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Apr 10, 2012·Bioconjugate Chemistry·Anil WaghBenedict Law
Aug 20, 2010·Angewandte Chemie·Florent SamainEric T Kool
May 8, 2013·Bioscience, Biotechnology, and Biochemistry·Akio KoboriAkira Murakami
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Aug 26, 2017·Journal of the American Chemical Society·Ke Min ChanEric T Kool

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