Polygalacturonase-inhibiting protein interacts with pectin through a binding site formed by four clustered residues of arginine and lysine.

Plant Physiology
Sara SpadoniDaniela Bellincampi

Abstract

Polygalacturonase-inhibiting protein (PGIP) is a cell wall protein that inhibits fungal polygalacturonases (PGs) and retards the invasion of plant tissues by phytopathogenic fungi. Here, we report the interaction of two PGIP isoforms from Phaseolus vulgaris (PvPGIP1 and PvPGIP2) with both polygalacturonic acid and cell wall fractions containing uronic acids. We identify in the three-dimensional structure of PvPGIP2 a motif of four clustered arginine and lysine residues (R183, R206, K230, and R252) responsible for this binding. The four residues were mutated and the protein variants were expressed in Pichia pastoris. The ability of both wild-type and mutated proteins to bind pectins was investigated by affinity chromatography. Single mutations impaired the binding and double mutations abolished the interaction, thus indicating that the four clustered residues form the pectin-binding site. Remarkably, the binding of PGIP to pectin is displaced in vitro by PGs, suggesting that PGIP interacts with pectin and PGs through overlapping although not identical regions. The specific interaction of PGIP with polygalacturonic acid may be strategic to protect pectins from the degrading activity of fungal PGs.

References

Sep 26, 1997·Molecular Plant-microbe Interactions : MPMI·A DesiderioF Cervone
Nov 1, 2001·Proceedings of the National Academy of Sciences of the United States of America·L FedericiD Tsernoglou
Nov 10, 2001·Annual Review of Phytopathology·G De LorenzoF Cervone
May 9, 2002·Journal of Experimental Botany·G Paul BolwellFarida Minibayeva
Jun 5, 2002·Annual Review of Biochemistry·Jeffrey D Esko, Scott B Selleck
Aug 9, 2003·Proceedings of the National Academy of Sciences of the United States of America·A Di MatteoF Cervone
Nov 7, 2003·Molecular Plant-microbe Interactions : MPMI·Gabré KempZacharias A Pretorius
Mar 17, 2005·Plant & Cell Physiology·Annabelle Decreux, Johan Messiaen
Jan 13, 2006·Trends in Plant Science·Luca FedericiFelice Cervone

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Citations

Jan 18, 2008·Proceedings of the National Academy of Sciences of the United States of America·D CantuA L T Powell
Apr 18, 2009·Proceedings of the National Academy of Sciences of the United States of America·Manuela CasasoliGiulia De Lorenzo
Jan 11, 2008·Molecular Plant-microbe Interactions : MPMI·Michela JanniRenato D'Ovidio
Mar 27, 2009·Plant Physiology·Yoshiharu SawakiHiroyuki Koyama
Nov 11, 2008·Biochemistry. Biokhimii︠a︡·M A ProtsenkoN P Korableva
Jul 25, 2014·Journal of Experimental Botany·Fabien SénéchalJérôme Pelloux
Jan 10, 2012·Annual Review of Plant Biology·Sebastian WolfHerman Höfte
Feb 25, 2009·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Anna FarinaRenato D'Ovidio
Sep 25, 2009·Journal of Proteomics·Vera MuccilliSalvatore Foti
Jun 6, 2009·Seminars in Cell & Developmental Biology·Stijn LagaertGuido Volckaert
Oct 1, 2008·Trends in Plant Science·Dario CantuAnn L T Powell
Jun 14, 2008·Current Opinion in Plant Biology·Johana C Misas-Villamil, Renier A L van der Hoorn
Jan 13, 2011·The Plant Journal : for Cell and Molecular Biology·Monica De CaroliGabriella Piro
Jul 27, 2010·Plant Signaling & Behavior·Volker BischoffWolf-Rüdiger Scheible
Jun 28, 2011·Plant Signaling & Behavior·Monica De CaroliGabriella Piro
Oct 8, 2014·Proceedings of the National Academy of Sciences of the United States of America·Sebastian WolfHerman Höfte
Jun 7, 2014·Frontiers in Plant Science·Daniela BellincampiVincenzo Lionetti
Jul 2, 2016·Molecular Plant-microbe Interactions : MPMI·Silvio TundoRenato D'Ovidio
Oct 21, 2017·Journal of Experimental Botany·Syed Jehangir ShahShahid Siddique
Feb 6, 2018·The Plant Journal : for Cell and Molecular Biology·Manuel BenedettiFelice Cervone
Jan 23, 2008·Proteomics·Elisabeth JametRafael Pont-Lezica
Feb 6, 2017·The Biochemical Journal·Sebastian Wolf
Aug 29, 2018·International Journal of Molecular Sciences·Annelie GutschKjell Sergeant
Dec 22, 2017·The Plant Journal : for Cell and Molecular Biology·Laura BaceteAntonio Molina
Oct 27, 2020·Frontiers in Plant Science·Liangjie NiuWei Wang

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