Polygalacturonase inhibiting protein: isolation, developmental regulation and pathogen related expression in Panax ginseng C.A. Meyer.

Molecular Biology Reports
Gayathri SathiyarajDeok-Chun Yang

Abstract

Polygalacturonase inhibiting proteins (PGIPs) are the major defense proteins which play an important role in resistance to infection of pathogens. A putative novel gene encoding PGIP was isolated from Panax ginseng C.A. Meyer, which shows 70.3 and 68.4% homology with chick pea and Arabidopsis PGIPs. The RACE PCR was preformed to isolate the full-length PGIP cDNA from Panax ginseng. Sequence analysis revealed that the cDNA of PgPGIP is of 1,275 bp in length and that it's containing ORF encodes for a polypeptide of 366 amino acids. Domain analysis revealed that the deduced amino acid sequences of PgPGIP have a typical PGIP topology. The transcription level of PgPGIP was up-regulated in ginseng in response to wounding and infection with phytopathogenic fungi i.e., Rhizoctonia solani, Fusarium oxysporum, Phythium ultimum, Botrytis cinerea, Colletotrichum gloeosporioides and Cylindrocarpon destructans, which causes drastic damage in ginseng plants. The constitutive PgPGIP expression of 4 years old plant, showed elevated transcript level, especially roots, showed maximum then buds, stems and leaves, indicating that the gene is developmentally regulated. The crude PGIP extracts derived from the fungal infected plants directly reduces ...Continue Reading

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Citations

Jul 20, 2011·Plant Cell Reports·Sathiyamoorthy SubramaniyamYang Deok Chun
Jun 14, 2018·Current Protein & Peptide Science·Yau Sang ChanTzi Bun Ng
Dec 14, 2011·Biochemistry. Biokhimii︠a︡·Balusamy Sri Renuka DeviDeok-Chun Yang
Jul 25, 2014·Journal of Experimental Botany·Fabien SénéchalJérôme Pelloux
Nov 19, 2010·Molecular Biology Reports·Gayathri SathiyarajDeok Chun Yang

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