Polygenic Analysis in Absence of Major Effector ATF1 Unveils Novel Components in Yeast Flavor Ester Biosynthesis

MBio
Sylvester HoltJohan M Thevelein

Abstract

Flavor production in yeast fermentation is of paramount importance for industrial production of alcoholic beverages. Although major enzymes of flavor compound biosynthesis have been identified, few specific mutations responsible for strain diversity in flavor production are known. The ATF1-encoded alcohol acetyl coenzyme A (acetyl-CoA) transferase (AATase) is responsible for the majority of acetate ester biosynthesis, but other components affecting strain diversity remain unknown. We have performed parallel polygenic analysis of low production of ethyl acetate, a compound with an undesirable solvent-like off-flavor, in strains with and without deletion of ATF1 We identified two unique causative mutations, eat1K179fs and snf8E148*, not present in any other sequenced yeast strain and responsible for most ethyl acetate produced in absence of ATF1EAT1 encodes a putative mitochondrial ethanol acetyl-CoA transferase (EATase) and its overexpression, but not that of EAT1K179fs , and strongly increases ethyl acetate without affecting other flavor acetate esters. Unexpectedly, a higher level of acetate esters (including ethyl acetate) was produced when eat1K179fs was present together with ATF1 in the same strain, suggesting that the Eat1...Continue Reading

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Citations

Nov 18, 2018·FEMS Microbiology Reviews·Sylvester HoltJohan M Thevelein
Feb 6, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Pei-Tong LiuGuo-Liang Yan
Jan 10, 2019·Frontiers in Microbiology·Aleksander J KruisRuud A Weusthuis
Nov 15, 2019·Journal of Agricultural and Food Chemistry·Niël van WykIsak S Pretorius
Feb 13, 2021·ACS Synthetic Biology·Wenqi ShiDongguang Xiao

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Datasets Mentioned

BETA
SRP107919

Methods Mentioned

BETA
Flow cytometry
PCR
acetylate

Software Mentioned

FastQC
CLC workbench
EXPLoRA
DNA
BLAST
R package PerformanceAnalytics
HMMER
Clustal Omega
Bowtie
R package Grofit

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