Polyglucosan Molecules Induce Mitochondrial Impairment and Apoptosis in Germ Cells Without Affecting the Integrity and Functionality of Sertoli Cells

Journal of Cellular Physiology
Franz Villarroel-EspíndolaJuan Carlos Slebe

Abstract

Glycogen is the main storage form of glucose; however, the accumulation of glycogen-like glucose polymers can lead to degeneration and cellular death. Previously, we reported that the accumulation of glycogen in testis of transgenic animals overexpressing a constitutively active form of glycogen synthase enhances the apoptosis of pre-meiotic male germ cells and a complete disorganization of the seminiferous tubules. Here we sought to further identify the effects of glycogen storage in cells from the seminiferous tubules and the mechanism behind the pro-apoptotic activity induced by its accumulation. Using an in vitro culture of Sertoli cells (line 42GPA9) and spermatocyte-like cells (line GC-1) expressing a superactive form of glycogen synthase or the Protein Targeting to Glycogen (PTG), we found that glycogen synthesized in both cell lines is poorly branched. In addition, the immunodetection of key molecules of apoptotic events suggests that cellular death induced by polyglucosan molecules affects GC-1 cells, but not 42GPA9 cells by mitochondrial impairment and activation of an intrinsic apoptotic pathway. Furthermore, we analyzed the effects of glycogen deposition during the establishment of an in vitro blood-testis barrier. ...Continue Reading

References

Jan 1, 1992·Canadian Journal of Physiology and Pharmacology·R A Swanson
Aug 1, 1992·Experimental Cell Research·M C HofmannJ L Millán
Jan 1, 1991·Methods in Enzymology·M J Hubbard, P Cohen
Apr 1, 1969·The Journal of Endocrinology·A FabbriniC Conti
Jan 1, 1993·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·R A Swanson, D W Choi
May 1, 1996·Human & Experimental Toxicology·P R DalsenterI Chahoud
Feb 25, 1998·Biology of Reproduction·V BourdonG Pointis
Oct 6, 2000·Andrologia·T K MonseesJ Hayatpour
Oct 24, 2000·Proceedings of the Society for Experimental Biology and Medicine·K BoekelheideH A Schoenfeld
Jul 1, 1962·Analytical Biochemistry·C R KRISMAN
Nov 14, 2003·Reproductive Toxicology·Sonu Chand ThakurShiv P Singh
Apr 15, 2004·Reproductive Toxicology·Céline FioriniGeorges Pointis
Jan 22, 2005·The Journal of Biological Chemistry·Abhijit GhoshJanice Yang Chou
Apr 7, 2006·Cell and Tissue Research·Ricardo D MorenoJuan G Reyes
Oct 15, 2008·The Journal of Biological Chemistry·Vincent S TagliabracciPeter J Roach
Feb 26, 2009·Toxicological Sciences : an Official Journal of the Society of Toxicology·Chikanori KuramoriHiroshi Handa
Oct 13, 2009·Trends in Biochemical Sciences·Matthew S GentryCarolyn A Worby
Apr 1, 2010·Acta Physiologica·T E GrahamR J Wilson
Apr 15, 2010·Proteomics·David StapletonGlendon Parker
Nov 3, 2010·Cell Metabolism·Michale BouskilaKei Sakamoto
Nov 16, 2010·Annals of Neurology·Julie TurnbullBerge A Minassian
Dec 17, 2010·Cancer Research·Eduardo N MaldonadoJohn J Lemasters
Mar 2, 2011·Cell Metabolism·Vincent S TagliabracciPeter J Roach
Jan 18, 2012·The Biochemical Journal·Peter J RoachVincent S Tagliabracci
May 3, 2012·EMBO Molecular Medicine·Jordi DuranJoan J Guinovart
Jul 26, 2014·Aging Cell·Christopher SinadinosJoan J Guinovart
Feb 15, 2015·Free Radical Biology & Medicine·Carlos Romá-MateoFederico V Pallardó

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Apoptosis

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