Poly(glycidyl methacrylate) beads embedded cryogels for pseudo-specific affinity depletion of albumin and immunoglobulin G

Materials Science & Engineering. C, Materials for Biological Applications
Nilay BereliAdil Denizli

Abstract

Depletion of high abundant proteins like albumin and immunoglobulin G (IgG) can be beneficial in the analysis of serum proteins. For this purpose, Cibacron Blue F3GA and iminodiacetic acid (IDA)-Cu2+ containing poly(glycidyl methacrylate) (PGMA) beads (1.6µm in diameter) were embedded into the poly(hydroxyethyl methacrylate) (PHEMA) cryogel. The PGMA beads were prepared by dispersion polymerization. The PGMA beads were modified with Cibacron Blue F3GA and iminodiacetic acid (IDA)-Cu2+ for simultaneous albumin and IgG depletion, respectively. The PHEMA cryogel was synthesized by free radical polymerization in the presence of the modified PGMA beads. The PHEMA and PHEMA/PGMA composite cryogels were characterized by swelling measurements and scanning electron microscopy (SEM). Protein depletion studies were carried out in a continuous experimental set-up in a stacked column. Albumin adsorption capacity of the PGMA-Cibacron Blue F3GA beads embedded PHEMA cryogel (PHEMA/PGMA-Cibacron Blue F3GA) was 342mg/g and IgG adsorption capacity of the PGMA-IDA-Cu2+ beads embedded PHEMA cryogel (PHEMA/PGMA-IDA-Cu2+) was 257mg/g. The composite cryogels depleted albumin and IgG from human serum with 89.4% and 93.6% efficiency, respectively. High ...Continue Reading

Citations

Sep 25, 2018·Biomedical Chromatography : BMC·Gisele Luiza PavanSonia Maria Alves Bueno
Apr 20, 2019·Gels·Yeşeren Saylan, Adil Denizli
May 8, 2013·Biotechnology Journal·Telma BarrosoAna Aguiar-Ricardo
Jan 12, 2013·Artificial Cells, Nanomedicine, and Biotechnology·Şeyda Ceylan, Mehmet Odabaşı
Dec 16, 2017·Journal of Biomaterials Science. Polymer Edition·Esra Feyzioğlu DemirSinan Akgöl
Oct 8, 2013·Artificial Cells, Nanomedicine, and Biotechnology·Öznur GökayAdil Denizli
May 16, 2014·Journal of Separation Science·Fatma Kartal, Adil Denizli
Dec 28, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Xue HuJian-Hua Wang
Aug 26, 2020·Chemical Communications : Chem Comm·Meng-Meng WangJian-Hua Wang
Oct 17, 2012·Biomedical Chromatography : BMC·Nilay BereliAdil Denizli
May 11, 2012·Applied Biochemistry and Biotechnology·Deniz Aktaş UygunAdil Denizli
Jan 3, 2015·Journal of Biomaterials Science. Polymer Edition·Murat UygunAdil Denizli
Oct 26, 2016·Artificial Cells, Nanomedicine, and Biotechnology·Kemal ÇetinAdil Denizli
Jun 8, 2011·Journal of Separation Science·Fatima M PlievaBo Mattiasson
Jun 4, 2010·Applied Biochemistry and Biotechnology·Nesibe AvcibaşiAdil Denizli
Jul 11, 2013·Applied Biochemistry and Biotechnology·Işık PerçinAdil Denizli
Jun 23, 2010·Journal of Biomaterials Science. Polymer Edition·Evrim Banu AltıntaşAdil Denizli
Nov 16, 2013·Applied Biochemistry and Biotechnology·Murat UygunSinan Akgöl
Jul 22, 2014·Journal of Molecular Recognition : JMR·Gözde BaydemirAdil Denizli
Jun 12, 2014·Journal of Chromatography. a·Gizem Ertürk, Bo Mattiasson
Mar 27, 2012·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Ali DoğanAdil Denizli

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