Polyhemoglobin with different percentage of tetrameric hemoglobin and effects on vasoactivity and electrocardiogram

Artificial Cells, Blood Substitutes, and Immobilization Biotechnology
Binglan YuThomas M S Chang

Abstract

There has been considerable discussions on why some types of haemoglobin-based blood substitutes increase vasoactivity whereas a very few others do not. In this study, we prepare four different types of PolyHb each containing different percentage of tetrameric hemoglobin using glutaraldehyde crosslinking and characterized to ensure that they all have the same oxygen affinity. Thus the preparations are prepared from the same chemical method and have the same oxygen affinity. We infused these in the form of 1/6 volume toploading into anesthetized rats to simulate the use of blood substitutes in surgery. Mean arterial pressure (MAP) increased immediately after injection of PolyHb containing 38% or 78% of tetrameric hemoglobin. However, there was no significant increase in blood pressure with the injection of PolyHb containing 16% or 0.4% tetrameric hemoglobin. In electrocardiogram (ECG) study, we observe that high percentage (78%) of tetrameric hemoglobin causes marked changes in ECG immediately after infusion. Injection of PolyHb containing 16% or 38% of tetrameric hemoglobin resulted in minimal elevation of the ST segment. Infusion of PolyHb containing 0.4% of tetrameric hemoglobin did not result in any changes.

References

Feb 9, 2000·Journal of Vascular Surgery·G M LaMuragliaE Vandermeersch
Dec 5, 2000·Baillière's Best Practice & Research. Clinical Haematology·T M Chang
Mar 31, 2001·Artificial Cells, Blood Substitutes, and Immobilization Biotechnology·A CaronP Menu
Sep 18, 2002·Journal of Applied Physiology·Barbara MathesonRaymond C Koehler
Oct 12, 2002·Journal of the American College of Surgeons·Steven A GouldGerald S Moss
Apr 19, 2003·Journal of Internal Medicine·R M Winslow
Apr 19, 2003·Journal of Internal Medicine·T M S Chang

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Citations

Jul 4, 2013·ASAIO Journal : a Peer-reviewed Journal of the American Society for Artificial Internal Organs·Pedro Cabrales, Marcos Intaglietta
Feb 21, 2009·Artificial Cells, Blood Substitutes, and Immobilization Biotechnology·Ada Stefanescu, Thomas Ming Swi Chang
Nov 9, 2006·Artificial Cells, Blood Substitutes, and Immobilization Biotechnology·Thomas Ming Swi Chang
Jul 4, 2006·Trends in Biotechnology·Thomas Ming Swi Chang
Jun 22, 2010·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Thomas Ming Swi Chang

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