Poly(I:C) increases the expression of mPGES-1 and COX-2 in rat primary microglia

Journal of Neuroinflammation
Antonio C P de OliveiraBernd L Fiebich

Abstract

Microglia recognize pathogen-associated molecular patterns such as double-stranded RNA (dsRNA) present in some viruses. Polyinosinic-polycytidylic acid [poly(I:C)] is a synthetic analog of dsRNA that activates different molecules, such as retinoic acid-inducible gene I, melanoma differentiation-associated gene 5, and toll-like receptor-3 (TLR3). Poly(I:C) increases the expression of different cytokines in various cell types. However, its role in the regulation of the production of inflammatory mediators of the arachidonic acid pathway by microglia is poorly understood. In the present study, we evaluated the effect of poly(I:C) on the production of prostaglandin E2 (PGE2) and the inducible enzymes cyclooxygenase-2 (COX-2) and microsomal prostaglandin E synthase-1 (mPGES-1) in primary rat microglia. Microglia were stimulated with different concentrations of poly(I:C) (0.1-10 μg/ml), and the protein levels of COX-2 and mPGES-1, as well as the release of PGE2, were determined by western blot and enzyme immunoassay (EIA), respectively. Values were compared using one-way ANOVA with post hoc Student-Newman-Keuls test. Poly(I:C) increased the production of PGE2, as well as mPGES-1 and COX-2 synthesis. To investigate the mechanisms invo...Continue Reading

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Citations

May 5, 2016·Scientific Reports·Paula Maria Quaglio BelloziAntônio Carlos Pinheiro de Oliveira
Feb 2, 2017·Journal of Neuroinflammation·Soraya Wilke SalibaAntonio Carlos Pinheiro de Oliveira
Sep 4, 2016·Naunyn-Schmiedeberg's Archives of Pharmacology·Yoon Jeong NamChung Soo Lee
Dec 15, 2017·Journal of Neuroinflammation·Soraya Wilke SalibaBernd L Fiebich
Oct 20, 2018·Frontiers in Cellular Neuroscience·Bernd L FiebichAntonio Carlos Pinheiro de Oliveira
Jul 28, 2020·Cells·José A Rodríguez-GómezMiguel A Burguillos

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