Polyketide synthases of Diaporthe helianthi and involvement of DhPKS1 in virulence on sunflower

BMC Genomics
Michelina RuoccoFelice Scala

Abstract

The early phases of Diaporthe helianthi pathogenesis on sunflower are characterized by the production of phytotoxins that may play a role in host colonisation. In previous studies, phytotoxins of a polyketidic nature were isolated and purified from culture filtrates of virulent strains of D. helianthi isolated from sunflower. A highly aggressive isolate (7/96) from France contained a gene fragment of a putative nonaketide synthase (lovB) which was conserved in a virulent D. helianthi population. In order to investigate the role of polyketide synthases in D. helianthi 7/96, a draft genome of this isolate was examined. We were able to find and phylogenetically analyse 40 genes putatively coding for polyketide synthases (PKSs). Analysis of their domains revealed that most PKS genes of D. helianthi are reducing PKSs, whereas only eight lacked reducing domains. Most of the identified PKSs have orthologs shown to be virulence factors or genetic determinants for toxin production in other pathogenic fungi. One of the genes (DhPKS1) corresponded to the previously cloned D. helianthi lovB gene fragment and clustered with a nonribosomal peptide synthetase (NRPS) -PKS hybrid/lovastatin nonaketide like A. nidulans LovB. We used DhPKS1 as a ...Continue Reading

References

May 1, 1991·Plant Molecular Biology·T Mozo, P J Hooykaas
Sep 22, 1998·Nature Biotechnology·M J de GrootA G Beijersbergen
Jun 29, 2000·Annual Review of Biochemistry·C KhoslaD E Cane
Apr 25, 2003·Nature·James E GalaganBruce Birren
Apr 26, 2003·Current Opinion in Chemical Biology·Ben Shen
Dec 17, 2003·Proceedings of the National Academy of Sciences of the United States of America·Scott KrokenB Gillian Turgeon
Sep 16, 2004·Chembiochem : a European Journal of Chemical Biology·Zhongshu SongThomas J Simpson TJ
Feb 23, 2005·Chemical Communications : Chem Comm·James W SimsEric W Schmidt
Jun 29, 2005·Mycopathologia·Mariarosaria VergaraGiovanni Vannacci
Mar 7, 2006·Applied and Environmental Microbiology·Iffa Gaffoor, Frances Trail
May 24, 2006·Journal of Biotechnology·Julia Schümann, Christian Hertweck
Aug 12, 2009·Proceedings of the National Academy of Sciences of the United States of America·Volker SchroeckhAxel A Brakhage
Sep 10, 2009·Molecular Plant-microbe Interactions : MPMI·Wanessa D WightJonathan D Walton
Mar 6, 2010·The Journal of Antibiotics·Isao Fujii
May 5, 2010·Nucleic Acids Research·Mickael GoujonRodrigo Lopez
Nov 17, 2010·Nature Reviews. Microbiology·Jason M Crawford, Craig A Townsend
Apr 18, 2012·Journal of Computational Biology : a Journal of Computational Molecular Cell Biology·Anton BankevichPavel A Pevzner
Jul 24, 2014·Natural Product Reports·Thomas J Simpson

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Citations

Oct 29, 2018·World Journal of Microbiology & Biotechnology·Jae Woo HanBeom Seok Kim
Mar 14, 2021·Journal of Microbiological Methods·Marina R A MontoyaAzucena Del Carmen Ridao

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Datasets Mentioned

BETA
AJ512137
MAVT00000000.2

Methods Mentioned

BETA
PCR
phosphotransferase

Software Mentioned

MAFFT
PhyML
BUSCO
BioEdit
SPAdes

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