Abstract
A type III polyketide synthase from Plumbago zeylanica (PzPKS) was cloned and expressed in tobacco plants to study whether the transgenic tobacco plants expressing PzPKS synthesize the pharmacologically important polyketide, plumbagin. High resolution mass spectrometry based metabolite profiling of two transgenic events and wild type tobacco plants was carried out to investigate changes in polyketides, including plumbagin. Ten polyketides, which included six pyrones and four naphthalene derivatives, were identified in PzPKS transgenic plants. While one pyrone, styryl-2-pyranone, was detected in both, wild type and transgenic tobacco plants, three pyrones were expressed only in the leaves of transgenic tobacco plants. The transgenic tobacco plants did not accumulate plumbagin, but showed accumulation of isoshinanolone in the roots, which is postulated to be the reduction product of plumbagin. In addition, leaves of transgenic tobacco plants accumulated 3-methyl-1,8-naphthalenediol, a postulated precursor of plumbagin. The results indicated the requirement of additional Plumbago-specific components in the biosynthetic pathway of this polyketide.
References
Aug 1, 1990·Plant Molecular Biology·R HainR Stöcker
Jul 30, 1999·Nature Structural Biology·J L FerrerJ P Noel
Sep 8, 2001·Natural Product Reports·J Staunton, K J Weissman
Sep 18, 2001·Journal of Experimental Botany·G Bringmann, D Feineis
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Oct 27, 2006·Plant Biology·G HeublH Meimberg
Jan 19, 2007·The FEBS Journal·Karin SpringobToni M Kutchan
Apr 21, 2007·Nature Protocols·Ric C H De VosRobert D Hall
Jun 15, 2007·Molecular Carcinogenesis·Sreekala NairM Radhakrishna Pillai
May 10, 2008·Phytochemistry·Aphacha JindaprasertToni M Kutchan
Sep 6, 2008·Journal of Pharmaceutical and Biomedical Analysis·Chen LiSheng-Xiang Jiang
Nov 22, 2008·Plant Biotechnology Journal·Bertrand DelaunoisPhilippe Jeandet
May 16, 2009·Journal of Experimental Botany·Kati HanhinevaSirpa O Kärenlampi
Feb 26, 2010·Clinical Pharmacology and Therapeutics·P B PhapaleY-R Yoon
Apr 2, 2010·Natural Product Reports·Ikuro Abe, Hiroyuki Morita
Jul 1, 2011·Plant Cell Reports·Zhongyu LiuShujin Zhao
Aug 28, 2012·Science·Jing-Ke WengClint Chapple
Sep 25, 2012·Methods in Enzymology·Jing-Ke Weng, Joseph P Noel
Jul 25, 2013·International Journal of Molecular Sciences·Philippe JeandetSylvain Cordelier
Citations
Apr 1, 1994·Journal of Ethnopharmacology·K R ShanmugasundaramE R Shanmugasundaram
May 14, 2020·Journal of Asian Natural Products Research·Seiichi SakamotoSatoshi Morimoto
Jul 19, 2017·Chembiochem : a European Journal of Chemical Biology·Johan Andersen-RanbergRubini Kannangara