Poly(l-lysine)-Coated Liquid Crystal Droplets for Sensitive Detection of DNA and Their Applications in Controlled Release of Drug Molecules

ACS Omega
Indu VermaSantanu Kumar Pal

Abstract

Interactions between DNA and adsorbed poly(l-lysine) (PLL) on liquid crystal (LC) droplets were investigated using polarizing optical microcopy and epi-fluorescence microscopy. Earlier, we demonstrated that adsorption of PLL to the LC/aqueous interface resulted in homeotropic orientation of the LC and thus exhibited a radial configuration of the LC confined within the droplets. Subsequent adsorption of DNA (single-stranded DNA/double-stranded DNA) at PLL-coated LC droplets was found to trigger an LC reorientation within the droplets, leading to preradial/bipolar configuration of those droplets. To our surprise, subsequent exposure of complementary ssDNA to ssDNA/adsorbed PLL-modified LC droplets did not cause the LC reorientation. This is likely due to the formation of polyplexes (DNA-PLL complex) as confirmed by fluorescence microscopy and atomic force microscopy. In addition, dsDNA-adsorbed PLL droplets have been found to be effectively useful to displace (controlled release) propidium iodide (a model drug) encapsulated within dsDNA over time. These observations suggest the potential for a label-free droplet-based LC detection system that can respond to DNA and may provide a simple method to develop DNA-based drug nanocarriers.

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Citations

Jun 12, 2019·Artificial Cells, Nanomedicine, and Biotechnology·Hanyang ZhangHui Jin
Jan 14, 2021·Nanoscale·Zhiyi YuanYu-Cheng Chen
Apr 21, 2021·Physical Chemistry Chemical Physics : PCCP·Pritam Kumar JanaLasse Laurson
May 13, 2021·Soft Matter·Ziyihui WangTiegen Liu
May 24, 2019·Langmuir : the ACS Journal of Surfaces and Colloids·Indu VermaSantanu Kumar Pal
Jan 31, 2019·Langmuir : the ACS Journal of Surfaces and Colloids·Xiuxiu YangZhongqiang Yang
Jul 23, 2020·Langmuir : the ACS Journal of Surfaces and Colloids·Guillaume DureyTeresa Lopez-Leon
Nov 3, 2020·ACS Nano·Yifan ZhangYu-Cheng Chen
Aug 14, 2020·Langmuir : the ACS Journal of Surfaces and Colloids·Abhijit DanAnwesha Sarkar
Dec 24, 2021·Chemical Reviews·Hari Krishna Bisoyi, Quan Li
Jan 11, 2022·Langmuir : the ACS Journal of Surfaces and Colloids·Oscar H Piñeres-QuiñonesClaribel Acevedo-Vélez

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Methods Mentioned

BETA
biosensors
biosensor
atomic force microscopy
AFM
transfection
fluorescence assay
Fluorescence
fluorescence imaging
electrophoresis

Software Mentioned

Origin Pro
FluorEssence

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