PMID: 3928377Sep 2, 1985Paper

Poly(L-proline)-binding proteins from chick embryos are a profilin and a profilactin

European Journal of Biochemistry
M Tanaka, H Shibata

Abstract

Two poly(L-proline)-binding proteins (PBP-1 and PBP-2) were purified from chick embryos by using a poly(L-proline)-agarose column. PBP-1 was composed of two different polypeptides (molecular masses of 42 kDa and 15 kDa). The molar ratio of the two proteins in the complex was 1:1. The other poly(L-proline)-binding protein, PBP-2, was the 15-kDa protein itself. The 42-kDa protein was confirmed to be an actin from the amino acid composition, by immunochemical evidence and by its ability to self-polymerize. In addition, the 42 + 15-kDa protein complex (PBP-1) inhibited DNase I, just as a monomeric actin did. The amino acid composition of the 15-kDa protein was similar to that of mammalian profilin and it inhibited the salt-induced polymerization of rabbit skeletal muscle actin. Therefore, we conclude that the two poly(L-proline)-binding proteins from the chick embryo are a profilactin and a profilin in chick embryo. The ability of profilactin to bind poly(L-proline) must be due to profilin itself, because the profilin has a greater affinity for poly(L-proline) than does profilactin. Additionally, both the monomeric and filamentous actin from rabbit skeletal muscle have no affinity for poly(L-proline).

References

Jan 1, 1979·Cold Spring Harbor Symposia on Quantitative Biology·H E VarmusJ M Bishop
May 1, 1979·FEBS Letters·L E NyströmR Jakes
Dec 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·E Lazarides, U Lindberg
Jul 1, 1973·Archives of Biochemistry and Biophysics·D Fujimoto, S Kanaya
Jan 1, 1972·Cold Spring Harbor Symposia on Quantitative Biology·R HuberW Steigemann
Sep 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·M ElzingaR S Adelstein
Dec 15, 1973·Journal of Molecular Biology·S Kanaya, D Fujimoto
Aug 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·R E Rhoads, S Udenfriend
Jun 1, 1968·Archives of Biochemistry and Biophysics·J J HuttonS Udenfriend
Jul 1, 1968·Archives of Biochemistry and Biophysics·J L Hedrick, A J Smith
Jan 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·M KleinK J Dorrington
Mar 1, 1983·International Journal of Peptide and Protein Research·T IsemuraK Sanada
Apr 1, 1980·European Journal of Biochemistry·I BlikstadS Eriksson
Feb 10, 1964·Biochimica Et Biophysica Acta·U LINBERG
Dec 28, 1964·Annals of the New York Academy of Sciences·B J DAVIS

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Citations

Dec 1, 1994·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·F S Southwick, D L Purich
Jan 1, 1993·Cell Motility and the Cytoskeleton·G Babcock, P A Rubenstein
Apr 18, 2000·Gene·D E Wilkes, J J Otto
Jan 1, 1995·Nature Structural Biology·D C AmbergD Botstein
May 7, 1999·European Journal of Biochemistry·D B OstranderJ A Gorman
Aug 10, 2000·European Journal of Biochemistry·N WittenmayerK Schlüter
Mar 21, 1996·The New England Journal of Medicine·F S Southwick, D L Purich
Dec 14, 2011·Proceedings of the National Academy of Sciences of the United States of America·Ying BaoJonathan F Wendel
Dec 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·R A BrundageD A Portnoy
May 24, 1994·Proceedings of the National Academy of Sciences of the United States of America·F S Southwick, D L Purich
Apr 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·S M Ahern-DjamaliF M Hoffmann
Aug 1, 1987·The Journal of Cell Biology·S E LindT P Stossel
Jun 1, 1991·The Journal of Cell Biology·P J Goldschmidt-ClermontT D Pollard
Dec 1, 1992·The Journal of Cell Biology·I SadlerM C Beckerle
Sep 1, 1993·The Journal of Cell Biology·V K VinsonD A Torchia
Feb 10, 2004·Molecular Biology of the Cell·Nina WittenmayerBrigitte M Jockusch
Jun 23, 1998·The Plant Cell·S R ClarkeV E Franklin-Tong
Jun 15, 1992·European Journal of Biochemistry·B Nefsky, A Bretscher
Jun 1, 1993·American Journal of Respiratory Cell and Molecular Biology·E L Bearer
Apr 6, 1999·The Journal of Cell Biology·I KarakesisoglouM Schleicher
Jul 3, 1998·European Journal of Cell Biology·K SchlüterB M Jockusch
May 11, 2004·The Journal of Eukaryotic Microbiology·David E Wilkes, Joann J Otto
Sep 19, 2008·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Andreas Birbach
Dec 22, 2005·The FEBS Journal·Malika BoukhelifaCarol A Otey
May 11, 2006·Journal of Structural Biology·David Chereau, Roberto Dominguez

❮ Previous
Next ❯

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