Poly(lactic-co -glycolic acid)-loaded nanoparticles of betulinic acid for improved treatment of hepatic cancer: characterization, in vitro and in vivo evaluations

International Journal of Nanomedicine
Pranesh KumarSudipta Saha

Abstract

The application of betulinic acid (B), a potent antineoplastic agent, is limited due to poor bioavailability, short plasma half-life and inappropriate tissue distribution. Thus, we aimed to prepare novel 50:50 poly(lactic-co-glycolic acid) (PLGA)-loaded B nanoparticles (BNP) and to compare its anti-hepatocellular carcinoma (HCC) activity with parent B. BNP were synthesized and characterized using different methods such as scanning electron microscopy (SEM), fourier-transform infrared (FTIR) spectrometry and particle size analyses. Particle size of BNP was optimized through the application of the stabilizer, polyvinyl alcohol (PVA). The anti-HCC response was evaluated through in vitro cell line study using Hep-G2 cells, confocal microscopy, in vivo oral pharmacokinetics and animal studies. Further, quantitative reverse transcription polymerase chain reaction (qRT-PCR) analysis was conducted to observe the changes in the expression of specific genes. Particle size of BNP was optimized through the application of the stabilizer, polyvinyl alcohol. Physicochemical characterization exhibited particle size of 257.1 nm with zeta potential -0.170 mV (optimized batch B, BNP). SEM and FTIR analyses of BNP showed that cylindrical particles...Continue Reading

Citations

Apr 24, 2019·The Journal of Pharmacology and Experimental Therapeutics·G KaurM N V Ravi Kumar
Sep 14, 2018·International Journal of Biomaterials·Firdos Alam KhanSarah Ameen Almofty
Jun 25, 2019·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Zhe MaZhidong Liu
Mar 7, 2021·Nanomaterials·Anna GrigorevaAlexey Pestryakov
Jul 26, 2019·Experimental Cell Research·Zhengqi ChengFanfan Zhou
Jul 17, 2021·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Mohamed Fawzi KabilIbrahim M El-Sherbiny
Aug 14, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Wenkai JiangWence Zhou
May 26, 2020·Current Molecular Pharmacology·Deepak PradhanGoutam Rath

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Methods Mentioned

BETA
scanning electron microscopy
enzyme-linked immunosorbent assay
ELISA
MDA

Software Mentioned

WinNonlin
GraphPad
FluoView
GraphPad Prism

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