PMID: 3756163Oct 9, 1986Paper

Polylysine induces pH-dependent fusion of acidic phospholipid vesicles: a model for polycation-induced fusion

Biochimica Et Biophysica Acta
A WalterR Blumenthal

Abstract

Polylysine induced aggregation and fusion of negatively charged small unilamellar phosphatidylcholine vesicles containing at least 10% anionic lipid. Aggregation was followed by absorbance changes and fusion was assayed both by electron microscopy and by fluorescence energy transfer between lipid probes. A method for preparing asymmetric vesicles, where the fluorescent probes were present only in the inner monolayer of the vesicle membrane, was developed. These vesicles were used to distinguish the inner and outer monolayer when measuring lipid mixing between vesicles. Since polylysine induced lipid mixing of both monolayers equally, fusion of these vesicles did occur. The extent of fusion was dependent on the charge ratio between bound polylysine and phosphatidylserine (PS) in the outer monolayer and was optimal at a ratio of about 1:1. Excess polylysine inhibited fusion. At a given concentration of polypeptide, fusion increased as the pH was lowered toward 3 with an apparent pKa near 4. Since this value is close to the pKa of the PS-carboxyl groups and far from the pKa of the lysine epsilon-amino groups, the pH dependence observed for fusion resides in the lipids rather than in the peptide. Fusion was dependent on the availab...Continue Reading

References

Sep 2, 1975·Biochimica Et Biophysica Acta·D PapahadjopoulosT Isac
Dec 15, 1977·Biochimica Et Biophysica Acta·J G Stollery, W J Vail
Jan 1, 1985·Biochemistry·P S Uster, D W Deamer
Oct 22, 1985·Biochemistry·A E GadS Nir
Dec 1, 1974·Chemistry and Physics of Lipids·I R Miller, D Bach
Aug 25, 1982·Biochimica Et Biophysica Acta·A E GadG D Eytan
Mar 9, 1983·Biochimica Et Biophysica Acta·A E Gad
Jul 27, 1983·Biochimica Et Biophysica Acta·K HongD Papahadjopoulos
Jul 7, 1981·Biochemistry·D K StruckR E Pagano
Dec 1, 1972·The Journal of Membrane Biology·M Montal

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Citations

May 1, 1990·Journal of Clinical Immunology·R A LevyM D Lockshin
Jan 1, 1991·Archives of Virology·P MastromarinoN Orsi
Oct 8, 1990·Biochimica Et Biophysica Acta·G Cevc
Apr 28, 1995·European Journal of Pharmacology·F Van BambekeM P Mingeot-Leclercq
Feb 1, 1995·Biophysical Journal·M P Sánchez-MigallónJ C Gómez-Fernández
Jun 6, 2000·Advanced Drug Delivery Reviews·E WagnerW Zauner
Nov 7, 2006·Biochimica Et Biophysica Acta·Dmitry VolodkinHelmuth Mohwald
May 1, 2009·Expert Review of Clinical Pharmacology·Mustafa AkbulutRobert K Prud'homme
May 20, 2009·Journal of Physics. Condensed Matter : an Institute of Physics Journal·F BordiD Truzzolillo
Aug 6, 1999·Gene Therapy·F ZhangJ Chroboczek
Dec 18, 2016·The Journal of Chemical Physics·Klemen BohincSylvio May
Nov 26, 2008·Glycobiology·G Dennis SprottHarold C Jarrell
Jan 1, 1986·Membrane Biochemistry·A WalterR Blumenthal
Jun 17, 2020·Journal of Colloid and Interface Science·Yehou M D GnopoDavid Putnam
Aug 11, 2020·Biophysical Chemistry·Virjinia Doltchinkova, Victoria Vitkova
Nov 26, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Dmitry A DavydovAlexander A Yaroslavov
Nov 18, 2000·Progress in Lipid Research·D C DrummondJ Leroux
Mar 1, 2008·The Journal of Physical Chemistry. B·Simona SennatoMassimo Cametti
Mar 6, 2003·Journal of the American Chemical Society·Fredric M MengerNickolay S Melik-Nubarov
Apr 5, 2012·Cold Spring Harbor Protocols·Sung Wan Kim

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