Polymer-albumin conjugate for the facilitated delivery of macromolecular platinum drugs

Macromolecular Rapid Communications
Aydan DagMartina H Stenzel

Abstract

The delivery of macromolecular platinum drugs into cancerous cells is enhanced by conjugating the polymer to albumin. The monomers N-(2-hydroxypropyl)methacrylamide (HPMA) and Boc protected 1,3-diaminopropan-2-yl acrylate (Ac-DAP-Boc) are copolymerized in the presence of a furan protected maleimide functionalized reversible addition-fragmentation chain transfer (RAFT) agent. The resulting polymer with a composition of P(HPMA14 -co-(Ac-DAP-Boc)9 ) and a molecular weight of Mn = 7600 g mol(-1) (Đ = 1.24) is used as a macromolecular ligand for the conjugation to the platinum drug. Thermogravimetric analysis reveals full conjugation. After deprotection of the maleimide functionality of the polymer, the reactive polymer is conjugated to albumin using the Cys34 functionality. The conjugation is monitored using size exclusion chromatography, MALDI-TOF (matrix assisted laser desorption ionization time-of-flight), and SDS Page (sodium dodecyl sulphate polyacrylamide gel electrophoresis). The polymer-albumin conjugates self-assemble in water into nanoparticles of sizes of around 80 nm thanks to the hydrophobic nature of the platinum drugs. The albumin coated nanoparticles are readily taken up by ovarian cancer cell lines and they show su...Continue Reading

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Citations

Jan 27, 2016·Biomacromolecules·Yanyan JiangMartina Heide Stenzel
Mar 8, 2016·Macromolecular Bioscience·Yanyan Jiang, Martina Stenzel
Feb 26, 2016·Chemical Society Reviews·Yi YanChuanbing Tang
Mar 30, 2017·Biomacromolecules·Hiteshri MakwanaCameron Alexander
Jan 21, 2019·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Chao TaoDong-An Wang
Dec 23, 2017·Macromolecular Bioscience·Yupeng WangYubin Huang
Aug 18, 2020·Journal of Controlled Release : Official Journal of the Controlled Release Society·Xinquan LiuDebadyuti Ghosh
Jul 30, 2021·Biomaterials Science·Caihong XianJun Wu

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