Polymer-based microfluidic chip for rapid and efficient immunomagnetic capture and release of Listeria monocytogenes

Lab on a Chip
L MalicTeodor Veres

Abstract

Infections caused by foodborne pathogens such as Listeria monocytogenes pose a threat to public health while timely detection is challenging due to pathogen low numbers. The development of robust and efficient sample preparation techniques is crucial to improve detection sensitivity and workflow. Immunomagnetic separation using magnetic nanoparticles (MNPs) is attractive, as it can efficiently capture target cells. For food safety applications, a platform is needed to rapidly process large sample volumes, allowing capture and release of target bacteria conjugated to immunomagnetic nanoparticles (IMNPs). Herein, we demonstrate a method for magnetic capture and release of bacteria-IMNPs complex based on a 3D magnetic trap integrated on a polymeric microfluidic device. The 3D magnetic capture region consist of a dense array of high-aspect ratio (3 : 1) cylindrical pillars embossed in thermoplastic polymer and coated with soft ferromagnetic nickel by an electroless deposition technique. This allows the generation of strong and switchable magnetic capture regions due to the very low remanence of the nickel shell. We propose and validate an optimized configuration of capture regions for efficient localized capture and rapid release o...Continue Reading

References

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Citations

Apr 27, 2017·Lab on a Chip·Javier Alejandro Hernández-CastroTeodor Veres
Dec 28, 2017·Advanced Healthcare Materials·Vanessa Fernandes CardosoSenentxu Lanceros-Mendez
Feb 20, 2019·Sensors·Jane Ru ChoiAlistair C Cowie
Aug 20, 2019·Biotechnology Progress·William G PittClifton M Anderson
Jun 19, 2021·Biosensors & Bioelectronics·Maite Garcia-HernandoFernando Benito-Lopez
Apr 16, 2020·Analytical Chemistry·Matthias GeisslerTeodor Veres
Mar 30, 2018·Analytical Chemistry·Dongxue ZhangLiang Qiao
Nov 13, 2019·Analytical Chemistry·Hyeonjeong LeeSangmin Jeon
Sep 12, 2019·ACS Applied Materials & Interfaces·Jingxing GuoHuangxian Ju

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