Polymer-Bioactive Glass Composite Filaments for 3D Scaffold Manufacturing by Fused Deposition Modeling: Fabrication and Characterization

Frontiers in Bioengineering and Biotechnology
Thomas DistlerAldo R Boccaccini

Abstract

Critical size bone defects are regularly treated by auto- and allograft transplantation. However, such treatments require to harvest bone from patient donor sites, with often limited tissue availability or risk of donor site morbidity. Not requiring bone donation, three-dimensionally (3D) printed implants and biomaterial-based tissue engineering (TE) strategies promise to be the next generation therapies for bone regeneration. We present here polylactic acid (PLA)-bioactive glass (BG) composite scaffolds manufactured by fused deposition modeling (FDM), involving the fabrication of PLA-BG composite filaments which are used to 3D print controlled open-porous and osteoinductive scaffolds. We demonstrated the printability of PLA-BG filaments as well as the bioactivity and cytocompatibility of PLA-BG scaffolds using pre-osteoblast MC3T3E1 cells. Gene expression analyses indicated the beneficial impact of BG inclusions in FDM scaffolds regarding osteoinduction, as BG inclusions lead to increased osteogenic differentiation of human adipose-derived stem cells in comparison to pristine PLA. Our findings confirm that FDM is a convenient additive manufacturing technology to develop PLA-BG composite scaffolds suitable for bone tissue engin...Continue Reading

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Citations

Feb 7, 2021·Materials Science & Engineering. C, Materials for Biological Applications·R HanP J Walsh
Mar 27, 2021·Biomedical Materials·Sathyan Vivekanand Anandhan, Uma Maheswari Krishnan
May 10, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Anna Diez-EscuderoNils P Hailer
Jul 27, 2021·Frontiers in Cell and Developmental Biology·Rayan FairagLisbet Haglund
Nov 2, 2021·Progress in Biomaterials·Parisa NikpourSayed Mahmood Rabiee

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Methods Mentioned

BETA
scanning electron microscopy
light microscopy
X-ray
PCR
infrared spectroscopy
Fluorescence microscopy
AFM

Software Mentioned

CTVox
NEXT
ImageJ
CFX Maestro
tinkercad
NRecon
Origin

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