Aug 1, 1977

Polymer cryoprotectants in the preservation of biological ultrastructure. I. Low temperature states of aqueous solutions of hydrophilic polymers

Journal of Microscopy
F FranksP Echlin

Abstract

The solid states formed by vitrified and frozen aqueous solutions of some hydrophilic polymers, able to act as biological cryoprotectants, have been studied by differential scanning calorimetry and freeze fracture electron microscopy. Glass transitions, devitrification, recrystallization and melting behaviour of aqueous solutions of polyvinylpyrrolidone, hydroxyethyl starch and dextran have been established. The vitrified polymer solutions exhibit a characteristic microspheral morphology which is not induced by the quench cooling process but is an inherent feature of the solutions themselves.

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Mentioned in this Paper

Science of Morphology
HAES-steril
Cryoprotective Effect
Electron Microscopy, Diagnostic
Povidone
Biologic Preservation
Polymers
Electron Microscopy
Hetastarch
Solutions

About this Paper

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