Polymer dots for quantifying the total hydrophobic pathogenic lysates in a single drop

Colloids and Surfaces. B, Biointerfaces
Hani Nasser Abdelhamid, Hui-Fen Wu

Abstract

A selective, rapid, small sample load (2-4 μL), and sensitive quantification method for the hydrophobic cellular biomolecules of pathogenic bacteria and their biosensing application were reported. The present approach is based on using polythiophene polymer dots (2.5 nm), which were prepared via the oxidation/polymerization reactions and then were characterized using transmission electron microscopy (TEM), Fourier transform infrared (FT-IR), ultraviolet spectroscopy (UV), and matrix (surface) assisted laser desorption/ionization mass spectrometry (M(S)ALDI-MS). The present method requires only gentle agitation for a single drop of aqueous bacteria suspension (10 μL, Pseudomonas aeruginosa (1×10(4) cfu/mL) and Staphylococcus aureus (1×10(5) cfu/mL)) with 1 mL of polythiophene (0.5 mg/mL) in chloroform, and the time required for quantifying the total hydrophobic was significantly reduced to less than 3 min. The polythiophene polymer dots is also a quantitative assessment of bacteria for aqueous and blood samples if exposed to more than 4-5 μL of pathogenic bacteria and thus, it is a new biosensor for quantitative hydrophobic portions. The fluorescence intensity of polythiophene was enhanced after adding different volumes of patho...Continue Reading

References

Mar 13, 2002·Biosensors & Bioelectronics·Manju GerardB D Malhotra
Nov 27, 2004·Chemistry : a European Journal·Hoang-Anh HoMario Leclerc
Dec 15, 2006·Chemical Reviews·John E Anthony
Feb 3, 2007·Journal of Chromatography. a·Jean-Pierre C L LacazeMichael A Quilliam
Mar 24, 2007·Chemical Reviews·Jana Zaumseil, Henning Sirringhaus
Mar 28, 2007·Chemical Reviews·Samuel W ThomasTimothy M Swager
Apr 13, 2007·Chemical Reviews·Amanda R Murphy, Jean M J Fréchet
Jan 18, 2008·Accounts of Chemical Research·Hoang-A HoMario Leclerc
Mar 11, 2008·Nature Reviews. Microbiology·Christopher Weidenmaier, Andreas Peschel
Mar 22, 2008·Angewandte Chemie·Sybille AllardUllrich Scherf
Mar 28, 2008·Chemistry : a European Journal·Beng S OngHualong Pan
Apr 15, 2008·Analytica Chimica Acta·Ulrich LangeVladimir M Mirsky
Jun 6, 2008·Journal of Chromatography. a·Xiang LiCheng Sun
Feb 12, 2009·ACS Nano·Changfeng WuJason McNeill
Jan 23, 2010·Journal of Microbiological Methods·Boris Wawrik, Brian H Harriman
Jun 11, 2010·Advanced Materials·Adrien Kaeser, Albertus P H J Schenning
Aug 6, 2010·Chemical Reviews·Johannes Pecher, Stefan Mecking
Oct 14, 2010·Analytical and Bioanalytical Chemistry·A RamanaviciusA Ramanaviciene
Oct 30, 2010·Biosensors & Bioelectronics·Shruti Nambiar, John T W Yeow
Jul 13, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Karolis LeonaviciusArunas Ramanavicius
Feb 22, 2012·Angewandte Chemie·Amaresh Mishra, Peter Bäuerle
Aug 30, 2012·Proteomics·Hui-Fen WuNazim Hasan
Dec 12, 2012·Nanotechnology·Ranulfo AllenGerald G Fuller
Feb 1, 2013·Beilstein Journal of Nanotechnology·Simon P Rittmeyer, Axel Groß
Feb 23, 2013·ACS Applied Materials & Interfaces·Chun Li, Gaoquan Shi
Mar 5, 2013·Analytica Chimica Acta·Habib BagheriMehrnoush Naderi
Mar 26, 2013·ACS Applied Materials & Interfaces·Marie-Pier PlanteMario Leclerc
May 25, 2013·Advanced Healthcare Materials·Libing LiuShu Wang

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Citations

Mar 15, 2016·Analytical and Bioanalytical Chemistry·Hani Nasser Abdelhamid, Hui-Fen Wu
Dec 11, 2014·Materials Science & Engineering. C, Materials for Biological Applications·Hani Nasser Abdelhamid, Hui-Fen Wu
Aug 7, 2014·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Mukesh L BhaisareHui-Fen Wu

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