Polymer dynamics in layer-by-layer assemblies of chitosan and heparin

Langmuir : the ACS Journal of Surfaces and Colloids
Maria LundinRobert D Tilton

Abstract

The layer-by-layer deposition method has been used to build a multilayer thin film with two polysaccharides, chitosan CH (weak polycation) and heparin HEP (strong polyanion), on planar quartz surfaces. The film structure and dynamics in aqueous solution were studied with fluorescence resonance energy transfer (FRET) and total internal reflection fluorescence (TIRF). Particular emphasis was placed on the effect of deposition conditions, i.e., pH and salt concentration, on the out-of-plane (vertical) diffusion of fluorescence labeled chitosan in the chitosan/heparin (CH/HEP) film. FRET analysis showed that CH molecules diffused within the film with a diffusion coefficient that was not significantly sensitive to the deposition pH and solution ionic strength. A pH-sensitive label bound to CH embedded within the CH/HEP film was sensitive to the charge of the outermost polymer layer even when buried under 14 alternate layers of CH and HEP. A consideration of the results obtained with both fluorescence techniques showed that the structure of the CH/HEP thin film was highly interpenetrated without clear boundaries between each layer. These results are consistent with the hypothesis that the previously observed exponential-like film gro...Continue Reading

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Citations

May 18, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Maria LundinEva Blomberg
Apr 10, 2013·Proceedings of the National Academy of Sciences of the United States of America·Jonathan B GilbertRobert E Cohen
Apr 23, 2014·Carbohydrate Polymers·Karoliina JunkaJanne Laine
May 5, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·G CadoF Boulmedais
Nov 9, 2010·Journal of Colloid and Interface Science·Zsombor FeldötöEva Blomberg
Jun 11, 2016·Colloids and Surfaces. B, Biointerfaces·Raquel TeixeiraIva Pashkuleva
Feb 19, 2019·Journal of Biomaterials Science. Polymer Edition·Irene CarmagnolaGianluca Ciardelli
Sep 14, 2014·Journal of Materials Chemistry. B, Materials for Biology and Medicine·C D EastonS MacNeil
Aug 26, 2010·Critical Reviews in Biotechnology·Simone S SilvaRui L Reis
May 7, 2017·Journal of Materials Chemistry. B, Materials for Biology and Medicine·A CarreteroI Pashkuleva
Dec 17, 2018·Carbohydrate Polymers·Miryam Criado-GonzalezRebeca Hernández
Jan 20, 2021·ACS Biomaterials Science & Engineering·Vittorio FerraraBruno Pignataro
Oct 12, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Emily D CranstonMark W Rutland

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