Polymer microarrays rapidly identify competitive adsorbents of virus-like particles

Biointerphases
Andrew J BlokMorgan R Alexander

Abstract

The emergence of SARS-CoV-2 highlights the global need for platform technologies to enable the rapid development of diagnostics, vaccines, treatments, and personal protective equipment (PPE). However, many current technologies require the detailed mechanistic knowledge of specific material-virion interactions before they can be employed, for example, to aid in the purification of vaccine components or in the design of a more effective PPE. Here, we show that an adaption of a polymer microarray method for screening bacterial-surface interactions allows for the screening of polymers for desirable material-virion interactions. Nonpathogenic virus-like particles including fluorophores are exposed to the arrays in an aqueous buffer as a simple model of virions carried to the surface in saliva/sputum. Competitive binding of Lassa and Rubella virus-like particles is measured to probe the relative binding properties of a selection of copolymers. This provides the first step in the development of a method for the discovery of novel materials with promise for viral binding, with the next being development of this method to assess absolute viral adsorption and assessment of the attenuation of the activity of live virus, which we propose w...Continue Reading

References

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Apr 12, 2019·ACS Combinatorial Science·Jingjing GongMark Bradley
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Jan 7, 2020·Analytical and Bioanalytical Chemistry·Elisa ChiodiMarcella Chiari
Jan 12, 2020·Materials Science & Engineering. C, Materials for Biological Applications·Antonio Conde-GonzálezMark Bradley

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Citations

Oct 13, 2020·Matter·Xuan XueMorgan R Alexander

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