Polymer Nanoparticle Engineering for Podocyte Repair: From in Vitro Models to New Nanotherapeutics in Kidney Diseases

ACS Omega
Claudio ColomboFrancesco Cellesi

Abstract

Specific therapeutic targeting of kidney podocytes, the highly differentiated ramified glomerular cells involved in the onset and/or progression of proteinuric diseases, could become the optimal strategy for preventing chronic kidney disease. With this aim, we developed a library of engineered polymeric nanoparticles (NPs) of tuneable size and surface properties and evaluated their interaction with podocytes. NP cytotoxicity, uptake, and cytoskeletal effects on podocytes were first assessed. On the basis of these data, nanodelivery of dexamethasone loaded into selected biocompatible NPs was successful in repairing damaged podocytes. Finally, a three-dimensional in vitro system of co-culture of endothelial cells and podocytes was exploited as a new tool for mimicking the mechanisms of NP interaction with glomerular cells and the repair of the kidney filtration barrier.

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Citations

Jun 6, 2019·American Journal of Physiology. Renal Physiology·Julie E Goodwin
Jun 10, 2020·Chemistry : a European Journal·Giulia NeriFrancesca Baldelli Bombelli
Feb 19, 2020·Journal of Controlled Release : Official Journal of the Controlled Release Society·Fatemeh OroojalianMichael R Hamblin
Mar 9, 2021·Future Medicinal Chemistry·Nimeet DesaiRakesh K Tekade
Jun 5, 2021·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Daniel Fleischmann, Achim Goepferich
Jun 15, 2021·Frontiers in Bioengineering and Biotechnology·Xiaohan HuangWeiqiang Lin

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Methods Mentioned

BETA
dynamic light scattering
fluorescence microscopy
Assay

Software Mentioned

AxioVision

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