Polymer-shielded dye-affinity chromatography

Journal of Molecular Recognition : JMR
B MattiassonN Garg

Abstract

Polymer-shielded dye-affinity chromatography is a form of chromatography in which the dye matrix forms complexes with a nonionic, water-soluble polymer such as poly(vinylpyrrolidone) or poly(vinyl alcohol), prior to the column chromatography of a crude protein extract, the idea being that polymer shielding of the dye will prevent nonspecific interactions between the target protein and the dye. The concept of polymer shielding and a strategy for the rational selection of polymers suitable as shielding agents are presented.

References

Feb 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·S T Thompson, E Stellwagen
Jun 1, 1991·Journal of Biotechnology·D Sii, A Sadana
Apr 11, 1986·Journal of Chromatography·R K Scopes
Apr 15, 1980·Biochemistry·R E BardenE A Dennis
Sep 1, 1980·European Journal of Biochemistry·D PomponF Lederer
Jul 1, 1980·The Biochemical Journal·R J Leatherbarrow, P D Dean
Nov 29, 2002·Journal of Chromatography. a·Pär ArvidssonBo Mattiasson

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Citations

Jul 17, 1999·Trends in Biotechnology·I Y Galaev, B Mattiasson
Sep 24, 1999·Biochimie·M DanielukJ Bandorowicz-Pikuła
Apr 11, 2009·Biotechnology Journal·A K Satheesh Babu, M A Vijayalakshmi
Mar 28, 2001·Journal of Chromatography. a·Y C LiB Mattiasson

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